AIMS: To make a quantitative evaluation of the differences in biofilm formation by Staphylococcus epidermidis using batch and fed-batch growth systems and to correlate this with production of the major biofilm polysaccharide, poly-N-acetyl glucosamine (PNAG). METHODS AND RESULTS: Dry weight measurements of biofilms formed in batch and fed-batch conditions were compared with haemagglutination titres, which measure the amount of PNAG produced. Strains grown in batch systems developed less biofilm than when grown in fed-batch systems. A good correlation was found between the amount of biofilm formed in fed-batch systems and the haemagglutination titres. CONCLUSIONS: Differences in biofilm formation and PNAG production by S. epidermidis are dependent on the availability of nutrients, with higher availability correlating with more biofilm and PNAG production. SIGNIFICANCE OF AND IMPACT OF THE STUDY: Comparisons of the formation of biofilms by S. epidermidis are dependent on choosing an appropriate biofilm growth system. Comparability or disparity of conclusions among different investigations will be strongly influenced by which mode S. epidermidis biofilms are formed.
AIMS: To make a quantitative evaluation of the differences in biofilm formation by Staphylococcus epidermidis using batch and fed-batch growth systems and to correlate this with production of the major biofilm polysaccharide, poly-N-acetyl glucosamine (PNAG). METHODS AND RESULTS: Dry weight measurements of biofilms formed in batch and fed-batch conditions were compared with haemagglutination titres, which measure the amount of PNAG produced. Strains grown in batch systems developed less biofilm than when grown in fed-batch systems. A good correlation was found between the amount of biofilm formed in fed-batch systems and the haemagglutination titres. CONCLUSIONS: Differences in biofilm formation and PNAG production by S. epidermidis are dependent on the availability of nutrients, with higher availability correlating with more biofilm and PNAG production. SIGNIFICANCE OF AND IMPACT OF THE STUDY: Comparisons of the formation of biofilms by S. epidermidis are dependent on choosing an appropriate biofilm growth system. Comparability or disparity of conclusions among different investigations will be strongly influenced by which mode S. epidermidis biofilms are formed.
Authors: Joseph G Joyce; Chitrananda Abeygunawardana; Qiuwei Xu; James C Cook; Robert Hepler; Craig T Przysiecki; Karen M Grimm; Keith Roper; Charlotte C Yu Ip; Leslie Cope; Donna Montgomery; Mason Chang; Sherilyn Campie; Martha Brown; Tessie B McNeely; Julie Zorman; Tomas Maira-Litrán; Gerald B Pier; Paul M Keller; Kathrin U Jansen; George E Mark Journal: Carbohydr Res Date: 2003-04-22 Impact factor: 2.104
Authors: E B H Hume; J Baveja; B Muir; T L Schubert; N Kumar; S Kjelleberg; H J Griesser; H Thissen; R Read; L A Poole-Warren; K Schindhelm; M D P Willcox Journal: Biomaterials Date: 2004-09 Impact factor: 12.479
Authors: Tomás Maira-Litrán; Andrea Kropec; C Abeygunawardana; Joseph Joyce; George Mark; Donald A Goldmann; Gerald B Pier Journal: Infect Immun Date: 2002-08 Impact factor: 3.441
Authors: Nuno Cerca; Kimberly K Jefferson; Tomas Maira-Litrán; Danielle B Pier; Casie Kelly-Quintos; Donald A Goldmann; Joana Azeredo; Gerald B Pier Journal: Infect Immun Date: 2007-04-30 Impact factor: 3.441