Literature DB >> 15466585

A single amino acid substitution converts gamma-glutamyltranspeptidase to a class IV cephalosporin acylase (glutaryl-7-aminocephalosporanic acid acylase).

Hideyuki Suzuki1, Chinatsu Miwa, Sayaka Ishihara, Hidehiko Kumagai.   

Abstract

The aspartyl residue at position 433 of gamma-glutamyltranspeptidase of Escherichia coli K-12 was replaced by an asparaginyl residue. This substitution enabled gamma-glutamyltranspeptidase to deacylate glutaryl-7-aminocephalosporanic acid, producing 7-aminocephalosporanic acid, which is a starting material for the synthesis of semisynthetic cephalosporins.

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Year:  2004        PMID： 15466585      PMCID： PMC522061          DOI： 10.1128/AEM.70.10.6324-6328.2004

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  16 in total

1.  Autocatalytic processing of gamma-glutamyltranspeptidase.

Authors:  Hideyuki Suzuki; Hidehiko Kumagai
Journal:  J Biol Chem       Date:  2002-08-31       Impact factor: 5.157

Review 2.  The use of enzymes in the chemical industry in Europe.

Authors:  Andreas Schmid; Frank Hollmann; Jin Byung Park; Bruno Bühler
Journal:  Curr Opin Biotechnol       Date:  2002-08       Impact factor: 9.740

3.  Molecular cloning of Escherichia coli K-12 ggt and rapid isolation of gamma-glutamyltranspeptidase.

Authors:  H Suzuki; H Kumagai; T Echigo; T Tochikura
Journal:  Biochem Biophys Res Commun       Date:  1988-01-15       Impact factor: 3.575

4.  gamma-Glutamyltranspeptidase from Escherichia coli K-12: purification and properties.

Authors:  H Suzuki; H Kumagai; T Tochikura
Journal:  J Bacteriol       Date:  1986-12       Impact factor: 3.490

5.  gamma-Glutamyltranspeptidase from Escherichia coli K-12: formation and localization.

Authors:  H Suzuki; H Kumagai; T Tochikura
Journal:  J Bacteriol       Date:  1986-12       Impact factor: 3.490

6.  Rapid and efficient site-specific mutagenesis without phenotypic selection.

Authors:  T A Kunkel
Journal:  Proc Natl Acad Sci U S A       Date:  1985-01       Impact factor: 11.205

7.  Method for determining whether a gene of Escherichia coli is essential: application to the polA gene.

Authors:  C M Joyce; N D Grindley
Journal:  J Bacteriol       Date:  1984-05       Impact factor: 3.490

8.  Cloning and random mutagenesis of the Erwinia herbicola tyrR gene for high-level expression of tyrosine phenol-lyase.

Authors:  T Katayama; H Suzuki; T Koyanagi; H Kumagai
Journal:  Appl Environ Microbiol       Date:  2000-11       Impact factor: 4.792

9.  The 2.0 A crystal structure of cephalosporin acylase.

Authors:  Y Kim; K Yoon; Y Khang; S Turley; W G Hol
Journal:  Structure       Date:  2000-10-15       Impact factor: 5.006

10.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205
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  3 in total

1.  Improvement of the glutaryl-7-aminocephalosporanic acid acylase activity of a bacterial gamma-glutamyltranspeptidase.

Authors:  Chiaki Yamada; Kyoko Kijima; Sayaka Ishihara; Chinatsu Miwa; Kei Wada; Toshihiro Okada; Keiichi Fukuyama; Hidehiko Kumagai; Hideyuki Suzuki
Journal:  Appl Environ Microbiol       Date:  2008-04-04       Impact factor: 4.792

2.  Evidence for conserved function of γ-glutamyltranspeptidase in Helicobacter genus.

Authors:  Mirko Rossi; Christian Bolz; Joana Revez; Sundus Javed; Nahed El-Najjar; Florian Anderl; Heidi Hyytiäinen; Pia Vuorela; Markus Gerhard; Marja-Liisa Hänninen
Journal:  PLoS One       Date:  2012-02-14       Impact factor: 3.240

3.  Bacterial γ-glutamyltranspeptidases, physiological function, structure, catalytic mechanism and application.

Authors:  Hideyuki Suzuki; Keiichi Fukuyama; Hidehiko Kumagai
Journal:  Proc Jpn Acad Ser B Phys Biol Sci       Date:  2020       Impact factor: 3.493
  3 in total

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