Literature DB >> 2892489

Molecular cloning of Escherichia coli K-12 ggt and rapid isolation of gamma-glutamyltranspeptidase.

H Suzuki1, H Kumagai, T Echigo, T Tochikura.   

Abstract

Based on the results of mapping of ggt, eight strains were selected from a gene library of E. coli. One of the strains harboring pLC9-12 was found to show 14 times higher gamma-glutamyltranspeptidase activity per cell than the wild type strain. The ggt was subcloned to the BamHI site of pUC18 and the recombinant plasmid pSH101 was obtained. Ggt- phenotype of gamma-glutamyltranspeptidase-deficient mutants was complemented by pSH101. The specific activity of the enzyme in cells harboring pSH101 was 37-fold higher than that in the wild type cells. gamma-Glutamyltranspeptidase was isolated from the periplasmic fraction of the cells by simple two steps and crystallized.

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Year:  1988        PMID： 2892489     DOI： 10.1016/0006-291x(88)90482-2

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  14 in total

1.  A single amino acid substitution converts gamma-glutamyltranspeptidase to a class IV cephalosporin acylase (glutaryl-7-aminocephalosporanic acid acylase).

Authors:  Hideyuki Suzuki; Chinatsu Miwa; Sayaka Ishihara; Hidehiko Kumagai
Journal:  Appl Environ Microbiol       Date:  2004-10       Impact factor: 4.792

2.  Evolutionary analysis of the two-component systems in Pseudomonas aeruginosa PAO1.

Authors:  Ying-Tsong Chen; Hwan You Chang; Chin Lung Lu; Hwei-Ling Peng
Journal:  J Mol Evol       Date:  2004-12       Impact factor: 2.395

Review 3.  Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Authors:  M K Berlyn
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

Review 4.  Linkage map of Escherichia coli K-12, edition 8.

Authors:  B J Bachmann
Journal:  Microbiol Rev       Date:  1990-06

5.  DNA sequence of the Escherichia coli K-12 gamma-glutamyltranspeptidase gene, ggt.

Authors:  H Suzuki; H Kumagai; T Echigo; T Tochikura
Journal:  J Bacteriol       Date:  1989-09       Impact factor: 3.490

6.  Cloning and nucleotide sequence of human gamma-glutamyl transpeptidase.

Authors:  E Rajpert-De Meyts; N Heisterkamp; J Groffen
Journal:  Proc Natl Acad Sci U S A       Date:  1988-12       Impact factor: 11.205

7.  Escherichia coli K-12 can utilize an exogenous gamma-glutamyl peptide as an amino acid source, for which gamma-glutamyltranspeptidase is essential.

Authors:  H Suzuki; W Hashimoto; H Kumagai
Journal:  J Bacteriol       Date:  1993-09       Impact factor: 3.490

8.  Clades of γ-glutamyltransferases (GGTs) in the ascomycota and heterologous expression of Colletotrichum graminicola CgGGT1, a member of the pezizomycotina-only GGT clade.

Authors:  Marco H Bello; Lynn Epstein
Journal:  J Microbiol       Date:  2013-03-02       Impact factor: 3.422

Review 9.  Functions of the gene products of Escherichia coli.

Authors:  M Riley
Journal:  Microbiol Rev       Date:  1993-12

10.  Use of bacterial gamma-glutamyltranspeptidase for enzymatic synthesis of gamma-D-glutamyl compounds.

Authors:  Hideyuki Suzuki; Shunsuke Izuka; Hiromichi Minami; Nobukazu Miyakawa; Sayaka Ishihara; Hidehiko Kumagai
Journal:  Appl Environ Microbiol       Date:  2003-11       Impact factor: 4.792
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