| Literature DB >> 15328889 |
Masahiro Watanabe1, Yoshihiro Kobashigawa, Tomoyasu Aizawa, Makoto Demura, Katsutoshi Nitta.
Abstract
The native and the molten globule states (N and MG states, respectively) of canine milk lysozyme (CML) were examined by CD spectroscopy and AGADIR algorithm, a helix-coil transition program. It revealed that the helical content of the MG state was higher than that of the N-state, suggesting that non-native alpha-helix is formed in the MG state of CML. Using AGADIR, it indicated the possibility of alpha-helix formation in the third beta-strand region in the MG state. To investigate this possibility, we designed a mutant, Q58P, in which the helical propensity of the MG state was significantly decreased around the third beta-strand region. It appeared that the absolute ellipticity value at 222 nm of the mutant in the MG state was smaller than that of the wild-type protein. It could be assumed that the non-native alpha-helix is formed around the third beta-strand region of wild-type CML in the MG state.Entities:
Mesh：
Substances：
Year: 2004 PMID： 15328889 DOI： 10.1023/b:jopc.0000032653.30096.41
Source DB: PubMed Journal: Protein J ISSN： 1572-3887 Impact factor: 2.371