Literature DB >> 15299355

Radiation damage in protein crystals at low temperature.

A Gonzalez1, C Nave.   

Abstract

This paper describes the study of the effects of radiation damage on the quality of data collected from a protein crystal at 100 K. It is shown that radiation damage causes measurable effects in the diffraction pattern. This implies that, even at liquid nitrogen temperatures, there is a limit to the size of a crystal from which a complete data set can be collected.

Entities:  

Year:  1994        PMID: 15299355     DOI: 10.1107/S0907444994006311

Source DB:  PubMed          Journal:  Acta Crystallogr D Biol Crystallogr        ISSN: 0907-4449


  15 in total

1.  Characterization of conditions required for X-Ray diffraction experiments with protein microcrystals.

Authors:  R Glaeser; M Facciotti; P Walian; S Rouhani; J Holton; A MacDowell; R Celestre; D Cambie; H Padmore
Journal:  Biophys J       Date:  2000-06       Impact factor: 4.033

2.  Global radiation damage at 300 and 260 K with dose rates approaching 1 MGy s⁻¹.

Authors:  Matthew Warkentin; Ryan Badeau; Jesse B Hopkins; Anne M Mulichak; Lisa J Keefe; Robert E Thorne
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2012-01-17

3.  Specific chemical and structural damage to proteins produced by synchrotron radiation.

Authors:  M Weik; R B Ravelli; G Kryger; S McSweeney; M L Raves; M Harel; P Gros; I Silman; J Kroon; J L Sussman
Journal:  Proc Natl Acad Sci U S A       Date:  2000-01-18       Impact factor: 11.205

4.  Strain relief at the active site of phosphoserine aminotransferase induced by radiation damage.

Authors:  Anatoly P Dubnovitsky; Raimond B G Ravelli; Alexander N Popov; Anastassios C Papageorgiou
Journal:  Protein Sci       Date:  2005-05-09       Impact factor: 6.725

5.  Can radiation damage to protein crystals be reduced using small-molecule compounds?

Authors:  Jan Kmetko; Matthew Warkentin; Ulrich Englich; Robert E Thorne
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2011-09-08

6.  Real-space analysis of radiation-induced specific changes with independent component analysis.

Authors:  Dominika Borek; Raquel Bromberg; Johan Hattne; Zbyszek Otwinowski
Journal:  J Synchrotron Radiat       Date:  2018-02-22       Impact factor: 2.616

7.  Radiation damage in macromolecular crystallography: what is it and why should we care?

Authors:  Elspeth F Garman
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2010-03-24

8.  The minimum crystal size needed for a complete diffraction data set.

Authors:  James M Holton; Kenneth A Frankel
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2010-03-24

9.  Experimental determination of the radiation dose limit for cryocooled protein crystals.

Authors:  Robin Leslie Owen; Enrique Rudiño-Piñera; Elspeth F Garman
Journal:  Proc Natl Acad Sci U S A       Date:  2006-03-20       Impact factor: 11.205

10.  Rapid X-ray photoreduction of dimetal-oxygen cofactors in ribonucleotide reductase.

Authors:  Kajsa G V Sigfridsson; Petko Chernev; Nils Leidel; Ana Popovic-Bijelic; Astrid Gräslund; Michael Haumann
Journal:  J Biol Chem       Date:  2013-02-11       Impact factor: 5.157

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