STUDY DESIGN: Posterolateral lumbar spine fusions in athymic rats. OBJECTIVES: To compare spine fusion rates of two different osteoinductive products. SUMMARY OF BACKGROUND DATA: Many osteoinductive bone graft alternatives are available. Grafton (a demineralized bone matrix [DBM]) and Osteogenic Protein-1 (OP-1, an individual recombinant bone morphogenetic protein) are two such alternatives. The relative efficacy of products from these two classes has not been previously studied. The athymic rat spine fusion model has been validated and demonstrated useful to minimize inflammatory responses to xenogeneic or differentially expressed proteins such as those presented by DBMs of human etiology. METHODS: Single-level intertransverse process fusions were performed in 60 athymic nude rats with 2 cc/kg of Grafton or OP-1 Putty. Half of each study group was killed at 3 weeks and half at 6 weeks. Fusion masses were assessed by radiography, manual palpation, and histology. RESULTS: At 3 weeks, manual palpation revealed a 13% fusion rate with Grafton and a 100% fusion rate with OP-1 (P = 0.0001). At 6 weeks, manual palpation revealed a 39% fusion rate of with Grafton and a 100% fusion rate with OP-1 (P = 0.0007). Similar fusion rates were found by histology at 3 and 6 weeks. Of note, one or two adjacent levels were fused in all of the OP-1 animals and none of the Grafton animals. CONCLUSIONS: Significant differences between the ability of Grafton and OP-1 to induce bone formation in an athymic rat posterolateral lumbar spine fusion model were found.
STUDY DESIGN: Posterolateral lumbar spine fusions in athymic rats. OBJECTIVES: To compare spine fusion rates of two different osteoinductive products. SUMMARY OF BACKGROUND DATA: Many osteoinductive bone graft alternatives are available. Grafton (a demineralized bone matrix [DBM]) and Osteogenic Protein-1 (OP-1, an individual recombinant bone morphogenetic protein) are two such alternatives. The relative efficacy of products from these two classes has not been previously studied. The athymic rat spine fusion model has been validated and demonstrated useful to minimize inflammatory responses to xenogeneic or differentially expressed proteins such as those presented by DBMs of human etiology. METHODS: Single-level intertransverse process fusions were performed in 60 athymic nude rats with 2 cc/kg of Grafton or OP-1 Putty. Half of each study group was killed at 3 weeks and half at 6 weeks. Fusion masses were assessed by radiography, manual palpation, and histology. RESULTS: At 3 weeks, manual palpation revealed a 13% fusion rate with Grafton and a 100% fusion rate with OP-1 (P = 0.0001). At 6 weeks, manual palpation revealed a 39% fusion rate of with Grafton and a 100% fusion rate with OP-1 (P = 0.0007). Similar fusion rates were found by histology at 3 and 6 weeks. Of note, one or two adjacent levels were fused in all of the OP-1 animals and none of the Grafton animals. CONCLUSIONS: Significant differences between the ability of Grafton and OP-1 to induce bone formation in an athymic rat posterolateral lumbar spine fusion model were found.
Authors: Ashvin K Dewan; Rahul A Dewan; Nathan Calderon; Angie Fuentes; Zawaunyka Lazard; Alan R Davis; Michael Heggeness; John A Hipp; Elizabeth A Olmsted-Davis Journal: J Orthop Surg Res Date: 2010-08-21 Impact factor: 2.359
Authors: Benjamin P Erickson; Allen R Pierce; Andrew K Simpson; John Nash; Jonathan N Grauer Journal: Clin Orthop Relat Res Date: 2008-01-03 Impact factor: 4.176
Authors: Neil Bhamb; Linda E A Kanim; Susan Drapeau; Suneeth Mohan; Erick Vasquez; Dan Shimko; William McKAY; Hyun W Bae Journal: Int J Spine Surg Date: 2019-10-31