Literature DB >> 1524496

Expression of the HIV-1 Nef protein in the baculovirus system: investigation of anti-Nef antibodies response in human sera and subcellular localization of Nef.

N Kienzle1, M Enders, M Buck, H Siakkou, S Jahn, G Petzold, K E Schneweis, M Bachmann, W E Müller, N Müller-Lantzsch.   

Abstract

The nef gene of HIV-1 was expressed in insect cells using the eucaryotic baculovirus system. The recombinant Nef protein frequently reacted with seropositive sera of HIV-1 and HIV-2 infected patients. Anti-Nef antibodies in HIV-1 seronegative high risk groups individuals were only occasionally seen. Confocal laser scanning microscopy demonstrated that Nef is present both in the cytoplasm and in the nucleus, indicating that Nef might directly function on gene expression.

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Year:  1992        PMID： 1524496     DOI： 10.1007/bf01309702

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  28 in total

1.  Biochemical characterization of Epstein-Barr virus nuclear antigen 2A.

Authors:  F A Grässer; P Haiss; S Göttel; N Mueller-Lantzsch
Journal:  J Virol       Date:  1991-07       Impact factor: 5.103

2.  Expression and cellular localization of the Nef protein from human immunodeficiency virus-1 in stably transfected B-cells.

Authors:  N Kienzle; M Bachmann; W E Müller; N Müller-Lantzsch
Journal:  Arch Virol       Date:  1992       Impact factor: 2.574

3.  Nucleotide sequence of the AIDS virus, LAV.

Authors:  S Wain-Hobson; P Sonigo; O Danos; S Cole; M Alizon
Journal:  Cell       Date:  1985-01       Impact factor: 41.582

4.  An efficient procedure for the isolation of recombinant baculovirus.

Authors:  J Pen; G W Welling; S Welling-Wester
Journal:  Nucleic Acids Res       Date:  1989-01-11       Impact factor: 16.971

5.  Shuttling of the autoantigen La between nucleus and cell surface after uv irradiation of human keratinocytes.

Authors:  M Bachmann; S Chang; H Slor; J Kukulies; W E Müller
Journal:  Exp Cell Res       Date:  1990-12       Impact factor: 3.905

6.  Antibody response to the viral negative factor (nef) in HIV-1 infection: a correlate of levels of HIV-1 expression.

Authors:  P Reiss; A de Ronde; J M Lange; F de Wolf; J Dekker; C Debouck; J Goudsmit
Journal:  AIDS       Date:  1989-04       Impact factor: 4.177

7.  Differential effects of nef on HIV replication: implications for viral pathogenesis in the host.

Authors:  C Cheng-Mayer; P Iannello; K Shaw; P A Luciw; J A Levy
Journal:  Science       Date:  1989-12-22       Impact factor: 47.728

8.  Identification of Epstein-Barr virus terminal protein 1 (TP1) in extracts of four lymphoid cell lines, expression in insect cells, and detection of antibodies in human sera.

Authors:  B Frech; U Zimber-Strobl; K O Suentzenich; O Pavlish; G M Lenoir; G W Bornkamm; N Mueller-Lantzsch
Journal:  J Virol       Date:  1990-06       Impact factor: 5.103

9.  Long latency precedes overt seroconversion in sexually transmitted human-immunodeficiency-virus infection.

Authors:  A Ranki; S L Valle; M Krohn; J Antonen; J P Allain; M Leuther; G Franchini; K Krohn
Journal:  Lancet       Date:  1987-09-12       Impact factor: 79.321

10.  Immunological variation and immunohistochemical localization of HIV-1 Nef demonstrated with monoclonal antibodies.

Authors:  V Ovod; A Lagerstedt; A Ranki; F O Gombert; R Spohn; M Tähtinen; G Jung; K J Krohn
Journal:  AIDS       Date:  1992-01       Impact factor: 4.177
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  4 in total

1.  Specific cleavage sites of Nef proteins from human immunodeficiency virus types 1 and 2 for the viral proteases.

Authors:  J Schorr; R Kellner; O Fackler; J Freund; J Konvalinka; N Kienzle; H G Kräusslich; N Mueller-Lantzsch; H R Kalbitzer
Journal:  J Virol       Date:  1996-12       Impact factor: 5.103

2.  Interleukin 10 is induced by recombinant HIV-1 Nef protein involving the calcium/calmodulin-dependent phosphodiesterase signal transduction pathway.

Authors:  E Brigino; S Haraguchi; A Koutsonikolis; G J Cianciolo; U Owens; R A Good; N K Day
Journal:  Proc Natl Acad Sci U S A       Date:  1997-04-01       Impact factor: 11.205

3.  Subcloning of HIV-2 nef genes in E. coli and immunological reactivity of expressed fusion proteins.

Authors:  R Ulrich; H Siakkou; J Mayer; N Kienzle; N Müller-Lantzsch; D H Krüger
Journal:  Virus Genes       Date:  1993-09       Impact factor: 2.332

4.  Fine mapping of HIV-1 Nef-epitopes by monoclonal antibodies.

Authors:  H Siakkou; S Jahn; N Kienzle; R Ulrich; C Grötzinger; T Schneider; B Kohleisen; G Pauli; R Spohn; G Jung
Journal:  Arch Virol       Date:  1993       Impact factor: 2.574
  4 in total

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