Literature DB >> 15243094

LightCycler-based differentiation of Mycobacterium abscessus and Mycobacterium chelonae.

L Sedlacek1, M Rifai, K Feldmann, F C Bange.   

Abstract

In this study we introduce a rapid procedure to identify Mycobacterium abscessus (types I and II) and M. chelonae using LightCycler-based analysis of the hsp65 gene. Results from 36 clinical strains were compared with hsp65 gene restriction analysis and biochemical profiles of bacilli. As all three methods yielded identical results for each isolate, this procedure offers an excellent alternative to previously established nucleic acid amplification-based techniques for the diagnosis of mycobacterial diseases.

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Year:  2004        PMID： 15243094      PMCID： PMC446237          DOI： 10.1128/JCM.42.7.3284-3287.2004

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

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2.  Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory.

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3.  Comparison of methods for Identification of Mycobacterium abscessus and M. chelonae isolates.

Authors:  M A Yakrus; S M Hernandez; M M Floyd; D Sikes; W R Butler; B Metchock
Journal:  J Clin Microbiol       Date:  2001-11       Impact factor: 5.948

4.  Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis.

Authors:  A Telenti; F Marchesi; M Balz; F Bally; E C Böttger; T Bodmer
Journal:  J Clin Microbiol       Date:  1993-02       Impact factor: 5.948

5.  Recovery of mycobacteria from patients with cystic fibrosis.

Authors:  F C Bange; P Kirschner; E C Böttger
Journal:  J Clin Microbiol       Date:  1999-11       Impact factor: 5.948

6.  hsp65 sequencing for identification of rapidly growing mycobacteria.

Authors:  H Ringuet; C Akoua-Koffi; S Honore; A Varnerot; V Vincent; P Berche; J L Gaillard; C Pierre-Audigier
Journal:  J Clin Microbiol       Date:  1999-03       Impact factor: 5.948

7.  Clinical features of pulmonary disease caused by rapidly growing mycobacteria. An analysis of 154 patients.

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9.  Infections with rapidly growing mycobacteria: report of 20 cases.

Authors:  Somnuek Sungkanuparph; Boonmee Sathapatayavongs; Roongnapa Pracharktam
Journal:  Int J Infect Dis       Date:  2003-09       Impact factor: 3.623

10.  Identification of clinically significant Mycobacterium fortuitum complex isolates.

Authors:  V A Silcox; R C Good; M M Floyd
Journal:  J Clin Microbiol       Date:  1981-12       Impact factor: 5.948
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  4 in total

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Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

2.  Real-Time PCR: Revolutionizing Detection and Expression Analysis of Genes.

Authors:  Sa Deepak; Kr Kottapalli; R Rakwal; G Oros; Ks Rangappa; H Iwahashi; Y Masuo; Gk Agrawal
Journal:  Curr Genomics       Date:  2007-06       Impact factor: 2.236

3.  Usefulness of three-channel multiplex real-time PCR and melting curve analysis for simultaneous detection and identification of the Mycobacterium tuberculosis complex and nontuberculous mycobacteria.

Authors:  Yun Ji Hong; Young Hoon Chung; Taek Soo Kim; Sang Hoon Song; Kyoung Un Park; Jae Joon Yim; Junghan Song; Jae Ho Lee; Eui Chong Kim
Journal:  J Clin Microbiol       Date:  2011-09-14       Impact factor: 5.948

4.  Characterization of rough and smooth morphotypes of Mycobacterium abscessus isolates from clinical specimens.

Authors:  Kai Rüger; Annegret Hampel; Sandra Billig; Nadine Rücker; Sebastian Suerbaum; Franz-Christoph Bange
Journal:  J Clin Microbiol       Date:  2013-11-06       Impact factor: 5.948
  4 in total

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