| Literature DB >> 15184184 |
Deepti Parashar1, D S Chauhan, V D Sharma, Aradhana Chauhan, S V S Chauhan, V M Katoch.
Abstract
For isolation of environmental mycobacteria, a decontamination procedure has been standardized by which treatment with 3% sodium dodecyl sulfate plus 4% NaOH (15 and 30 min for rapid and slow growers, respectively) is followed by incubation with 2% cetrimide (5 and 15 min for fast- and slow-growing mycobacteria, respectively); this procedure was found to completely eliminate contamination with other organisms and resulted in the isolation of only mycobacteria.Entities:
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Year: 2004 PMID: 15184184 PMCID: PMC427771 DOI: 10.1128/AEM.70.6.3751-3753.2004
Source DB: PubMed Journal: Appl Environ Microbiol ISSN: 0099-2240 Impact factor: 4.792