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Literature DB >> 1518387

Chemiluminescence detection of 8a-hydroperoxy-tocopherone in photooxidized alpha-tocopherol.

Abstract

Photosensitized oxidation products of alpha-tocopherol were analyzed by reversed phase high-performance liquid chromatography (CL-HPLC) in combination with chemiluminescence detection, to measure hydroperoxide derivatives. An intense peak was observed on the chemiluminescence chromatogram, corresponding to 8a-hydroperoxy-alpha-tocopherone (alpha-tocopherol hydroperoxide), the identity of which was confirmed by 1H nuclear magnetic resonance and mass spectrometry. Observed peak intensities correlated with the peroxide value of oxidized alpha-tocopherol. As little as 50 pmol of 8a-hydroperoxy-alpha-tocopherone was detectable. The CL-HPLC method, which is specific for the detection of hydroperoxides, should prove useful in studies on tocopherol oxidation in foods and biological systems.

Entities: Chemical

Mesh：

Substances：

Year: 1992 PMID： 1518387 DOI： 10.1007/bf02536478

Source DB: PubMed Journal: Lipids ISSN： 0024-4201 Impact factor: 1.880

14 in total

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Journal: Lipids Date: 1974-11 Impact factor: 1.880

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Journal: Lipids Date: 1990-09 Impact factor: 1.880

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Journal: Biochim Biophys Acta Date: 1980-01-03

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Journal: Lipids Date: 1989-03 Impact factor: 1.880

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Authors: P E Hughes; S B Tove
Journal: J Biol Chem Date: 1980-08-10 Impact factor: 5.157

4 in total

1. Analysis of the addition products of alpha-tocopherol with phosphatidylcholine-peroxyl radicals by high-performance liquid chromatography with chemiluminescent detection.

Authors: R Yamauchi; Y Hara; H Murase; K Kato
Journal: Lipids Date: 2000-12 Impact factor: 1.880