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Literature DB >> 1515365

DNA extraction from mixtures of body fluid using mild preferential lysis.

Abstract

A modification to the DNA extraction method "preferential lysis" (Gill et al. 1985) is proposed which can be applied to DNA mixtures of vaginal cells and spermatozoa. In mixtures with a low sperm content the further loss of sperm DNA caused by the extraction can be avoided by using "mild preferential lysis". Amplification by PCR (polymerase chain reaction) then yields sufficient DNA to be able to identify both components in the mixture.

Mesh：

Substances：
DNA

Year: 1992 PMID： 1515365 DOI： 10.1007/bf01369558

Source DB: PubMed Journal: Int J Legal Med ISSN： 0937-9827 Impact factor: 2.686

5 in total

1. Analysis of the VNTR locus D1S80 by the PCR followed by high-resolution PAGE.

Authors: B Budowle; R Chakraborty; A M Giusti; A J Eisenberg; R C Allen
Journal: Am J Hum Genet Date: 1991-01 Impact factor: 11.025

2. Population and family data of RFLP's using selected single- and multi-locus systems.

Authors: B Brinkmann; S Rand; P Wiegand
Journal: Int J Legal Med Date: 1991-03 Impact factor: 2.686

3. Molecular characterization of a spontaneously generated new allele at a VNTR locus: no exchange of flanking DNA sequence.

Authors: R K Wolff; Y Nakamura; R White
Journal: Genomics Date: 1988-11 Impact factor: 5.736

4. Amplification of a highly polymorphic VNTR segment by the polymerase chain reaction.

Authors: G T Horn; B Richards; K W Klinger
Journal: Nucleic Acids Res Date: 1989-03-11 Impact factor: 16.971

5. Forensic application of DNA 'fingerprints'.

Authors: P Gill; A J Jeffreys; D J Werrett
Journal: Nature Date: 1985 Dec 12-18 Impact factor: 49.962

5 in total

12 in total

Review 1. Human identity testing with PCR-based systems.

Authors: C Hohoff; B Brinkmann
Journal: Mol Biotechnol Date: 1999-12-01 Impact factor: 2.695

2. Separation of sperm and epithelial cells based on the hydrodynamic effect for forensic analysis.

Authors: Weiran Liu; Weixing Chen; Ran Liu; Yuan Ou; Haoran Liu; Lan Xie; Ying Lu; Caixia Li; Bin Li; Jing Cheng
Journal: Biomicrofluidics Date: 2015-08-31 Impact factor: 2.800

3. Automatic detection of spermatozoa for laser capture microdissection.

Authors: Mado Vandewoestyne; David Van Hoofstat; Filip Van Nieuwerburgh; Dieter Deforce
Journal: Int J Legal Med Date: 2008-07-26 Impact factor: 2.686

4. Population data and forensic efficiency values for the STR systems HumVWA, HumMBP and HumFABP.

Authors: A Möller; P Wiegand; C Grüschow; S A Seuchter; M P Baur; B Brinkmann
Journal: Int J Legal Med Date: 1994 Impact factor: 2.686

5. Method to predict the chance of developing a male profile out of mixtures of male and female DNA.

Authors: Patrick Johannes Laberke; Rahel Grossenbacher; Roland Hausmann; Beate Balitzki
Journal: Int J Legal Med Date: 2011-09-08 Impact factor: 2.686

6. High-resolution vertical PAGE: an alternative electrophoretic system with multiple forensic applications.

Authors: H R Schneider; S Rand
Journal: Int J Legal Med Date: 1996 Impact factor: 2.686

7. The validation of a 7-locus multiplex STR test for use in forensic casework. (II), Artefacts, casework studies and success rates.

Authors: R Sparkes; C Kimpton; S Gilbard; P Carne; J Andersen; N Oldroyd; D Thomas; A Urquhart; P Gill
Journal: Int J Legal Med Date: 1996 Impact factor: 2.686