Literature DB >> 15131220

Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnosis of SARS coronavirus pneumonia.

Patrick C Y Woo1, Susanna K P Lau, Beatrice H L Wong, Hoi-wah Tsoi, Ami M Y Fung, Kwok-hung Chan, Victoria K P Tam, J S Malik Peiris, Kwok-yung Yuen.   

Abstract

We report the evaluation of recombinant severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) nucleocapsid protein enzyme-linked immunosorbent assay (ELISA)-based antibody tests for serodiagnosis of SARS-CoV pneumonia and compare the sensitivities and specificities of this ELISA for detection of immunoglobulin G (IgG), IgM, IgA, and their combinations with serum samples from 149 healthy blood donors who donated blood 3 years ago as controls and 106 SARS-CoV pneumonia patients in Hong Kong. The specificities of the ELISA for IgG, IgM, and IgA detection were 95.3, 96.6, and 96.6%, respectively, with corresponding sensitivities of 94.3, 59.4, and 60.4%, respectively. The present ELISA appears to be a sensitive test for serodiagnosis of SARS-CoV pneumonia, is much more economical and less labor-intensive than the indirect immunofluorescence assay, and does not require cultivation of SARS-CoV.

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Year:  2004        PMID： 15131220      PMCID： PMC404667          DOI： 10.1128/JCM.42.5.2306-2309.2004

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  23 in total

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Journal:  Science       Date:  2003-05-01       Impact factor: 47.728

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Journal:  N Engl J Med       Date:  2003-04-10       Impact factor: 91.245

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8.  Localization of linear B-cell epitopes on infectious bronchitis virus nucleocapsid protein.

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Journal:  Vet Microbiol       Date:  2000-07-03       Impact factor: 3.293

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10.  Coronavirus as a possible cause of severe acute respiratory syndrome.

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  64 in total

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2.  Immunofluorescence assay for detection of the nucleocapsid antigen of the severe acute respiratory syndrome (SARS)-associated coronavirus in cells derived from throat wash samples of patients with SARS.

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3.  Monoclonal antibody-based antigen capture enzyme-linked immunosorbent assay reveals high sensitivity of the nucleocapsid protein in acute-phase sera of severe acute respiratory syndrome patients.

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4.  Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia.

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Journal:  J Clin Microbiol       Date:  2005-07       Impact factor: 5.948

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9.  Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in sars patients by enzyme-linked immunosorbent assay.

Authors:  Susanna K P Lau; Patrick C Y Woo; Beatrice H L Wong; Hoi-Wah Tsoi; Gibson K S Woo; Rosana W S Poon; Kwok-Hung Chan; William I Wei; J S Malik Peiris; Kwok-Yung Yuen
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