Literature DB >> 23616462

Detection of specific antibodies against tembusu virus in ducks by use of an E protein-based enzyme-linked immunosorbent assay.

Xiuchen Yin1, Rang Lv, Xiaodan Chen, Ming Liu, Ronghong Hua, Yun Zhang.   

Abstract

We developed an enzyme-linked immunosorbent assay (ELISA) using eukaryotically expressed E protein as the antigen (termed E-ELISA) to detect antibodies to tembusu virus (TMUV) in ducks. The E-ELISA did not react with antisera to other known pathogens, indicating the E protein is specific for recognizing anti-TMUV antibodies. Compared to the serum neutralization test, the specificity and sensitivity of the E-ELISA was 93.2 and 97.8%, respectively. Therefore, this E-ELISA is a sensitive and rapid method for detecting antibodies against TMUV in ducks.

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Year:  2013        PMID： 23616462      PMCID： PMC3697666          DOI： 10.1128/JCM.00361-13

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  21 in total

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Journal:  J Clin Microbiol       Date:  1979-10       Impact factor: 5.948

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Journal:  J Clin Microbiol       Date:  2002-06       Impact factor: 5.948

Review 4.  Antibody prophylaxis and therapy for flavivirus encephalitis infections.

Authors:  J T Roehrig; L A Staudinger; A R Hunt; J H Mathews; C D Blair
Journal:  Ann N Y Acad Sci       Date:  2001-12       Impact factor: 5.691

5.  Standardization of immunoglobulin M capture enzyme-linked immunosorbent assays for routine diagnosis of arboviral infections.

Authors:  D A Martin; D A Muth; T Brown; A J Johnson; N Karabatsos; J T Roehrig
Journal:  J Clin Microbiol       Date:  2000-05       Impact factor: 5.948

6.  West Nile virus recombinant DNA vaccine protects mouse and horse from virus challenge and expresses in vitro a noninfectious recombinant antigen that can be used in enzyme-linked immunosorbent assays.

Authors:  B S Davis; G J Chang; B Cropp; J T Roehrig; D A Martin; C J Mitchell; R Bowen; M L Bunning
Journal:  J Virol       Date:  2001-05       Impact factor: 5.103

7.  Performance of immunoglobulin G (IgG) and IgM enzyme-linked immunosorbent assays using a West Nile virus recombinant antigen (preM/E) for detection of West Nile virus- and other flavivirus-specific antibodies.

Authors:  Wayne R Hogrefe; Ronald Moore; Mary Lape-Nixon; Michael Wagner; Harry E Prince
Journal:  J Clin Microbiol       Date:  2004-10       Impact factor: 5.948

8.  Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnosis of SARS coronavirus pneumonia.

Authors:  Patrick C Y Woo; Susanna K P Lau; Beatrice H L Wong; Hoi-wah Tsoi; Ami M Y Fung; Kwok-hung Chan; Victoria K P Tam; J S Malik Peiris; Kwok-yung Yuen
Journal:  J Clin Microbiol       Date:  2004-05       Impact factor: 5.948

9.  Diagnosis of tick-borne encephalitis by a mu-capture immunoglobulin M-enzyme immunoassay based on secreted recombinant antigen produced in insect cells.

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Journal:  J Clin Microbiol       Date:  2003-09       Impact factor: 5.948

10.  Development of a blocking ELISA for detection of serum neutralizing antibodies against newly emerged duck Tembusu virus.

Authors:  Xuesong Li; Guoxin Li; Qiaoyang Teng; Lei Yu; Xiaogang Wu; Zejun Li
Journal:  PLoS One       Date:  2012-12-31       Impact factor: 3.240
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  6 in total

1.  Kinetic analysis of IgM monoclonal antibodies for determination of dengue sample concentration using SPR technique.

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Journal:  Bioengineered       Date:  2016-08-17       Impact factor: 3.269

2.  Duck tembusu virus and its envelope protein induce programmed cell death.

Authors:  Wulin Shaozhou; Chenxi Li; Qingshan Zhang; Runzhe Meng; Youlan Gao; Hongyu Liu; Xiaofei Bai; Yuhuan Chen; Ming Liu; Siguo Liu; Yun Zhang
Journal:  Virus Genes       Date:  2015-06-09       Impact factor: 2.332

3.  Advanced uracil DNA glycosylase-supplemented real-time reverse transcription loop-mediated isothermal amplification (UDG-rRT-LAMP) method for universal and specific detection of Tembusu virus.

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Journal:  Sci Rep       Date:  2016-06-07       Impact factor: 4.379

Review 4.  Structures and Functions of the Envelope Glycoprotein in Flavivirus Infections.

Authors:  Xingcui Zhang; Renyong Jia; Haoyue Shen; Mingshu Wang; Zhongqiong Yin; Anchun Cheng
Journal:  Viruses       Date:  2017-11-13       Impact factor: 5.048

5.  Development and application of a monoclonal antibody-based blocking ELISA for detection of antibodies to Tembusu virus in multiple poultry species.

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Journal:  BMC Vet Res       Date:  2018-06-25       Impact factor: 2.741

6.  Detection of Neutralizing Antibodies to Tembusu Virus: Implications for Infection and Immunity.

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Journal:  Front Vet Sci       Date:  2019-12-10
  6 in total

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