| Literature DB >> 15123244 |
Jayaram Srinivasan1, Sharon T Cload, Nobuko Hamaguchi, Jeffrey Kurz, Sara Keene, Markus Kurz, Ryan M Boomer, Jill Blanchard, David Epstein, Charles Wilson, John L Diener.
Abstract
Two molecular sensors that specifically recognize ADP in a background of over 100-fold molar excess of ATP are described. These sensors are nucleic-acid based and comprise a general method for monitoring protein kinase activity. The ADP-aptamer scintillation proximity assay is configured in a single-step, homogeneous format while the allosteric ribozyme (RiboReporter) sensor generates a fluorescent signal upon ADP-dependent ribozyme self-cleavage. Both systems perform well when configured for high-throughput screening and have been used to rediscover a known protein kinase inhibitor in a high-throughput screening format.Entities:
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Year: 2004 PMID： 15123244 DOI： 10.1016/j.chembiol.2004.03.014
Source DB: PubMed Journal: Chem Biol ISSN： 1074-5521