| Literature DB >> 15112998 |
Carlos Olano1, Barrie Wilkinson, César Sánchez, Steven J Moss, Rose Sheridan, Vidya Math, Alison J Weston, Alfredo F Braña, Christine J Martin, Markiyan Oliynyk, Carmen Méndez, Peter F Leadlay, José A Salas.
Abstract
The biosynthetic gene cluster for the angiogenesis inhibitor borrelidin has been cloned from Streptomyces parvulus Tü4055. Sequence analysis indicates that the macrolide ring of borrelidin is formed by a modular polyketide synthase (PKS) (borA1-A6), a result that was confirmed by disruption of borA3. The borrelidin PKS is striking because only seven rather than the nine modules expected for a nonaketide product are encoded by borA1-A6. The starter unit of the PKS has been verified as trans-cyclopentane-1,2-dicarboxylic acid (trans-1,2-CPDA), and the genes involved in its biosynthesis identified. Other genes responsible for biosynthesis of the nitrile moiety, regulation, and self-resistance were also identified.Entities:
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Year: 2004 PMID： 15112998 DOI： 10.1016/j.chembiol.2003.12.018
Source DB: PubMed Journal: Chem Biol ISSN： 1074-5521