| Literature DB >> 150536 |
M Holsters, B Silva, F Van Vliet, J P Hernalsteens, C Genetello, M Van Montagu, J Schell.
Abstract
The Ti-plasmids are naturally self-transmissible from their normal host Agrobacterium to E. coli. They are however unable to stably establish themselves as a replicon in E. coli. It is nevertheless possible to study the Ti-plasmids in E. coli with the help of Ti::RP4 cointegrate plasmids that transfer and maintain themselves very efficiently in E. coli. An E. coli harbouring such a Ti::RP4 plasmid is unable to catabolize octopine and unable to induce crown-gall tumours on plants.Entities:
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Year: 1978 PMID: 150536 DOI: 10.1007/bf00271963
Source DB: PubMed Journal: Mol Gen Genet ISSN: 0026-8925