Literature DB >> 7038628

A coupled transcription-translation system from Agrobacterium tumefaciens and its application to study Ti plasmid expression in vitro.

T Kartasova, H Huisman, R Schilperoort.   

Abstract

A coupled transcription-translation system was isolated from A. tumefaciens. Expression of plasmids pBR322 and pKT212 from E.coli, cloned fragments of Ti plasmid (plasmids pSS155 and pSS156) and Ti plasmid derivatives pAL2802, pAL2811, pAL2821 and pAL2832 was analysed in an A. tumefaciens cell-free system and compared with their expression in an E.coli cell-free system. New proteins of 41K and 44K appeared in A tumefaciens extracts, as the result of Ti plasmid expression. These proteins were not found in E.coli extracts.

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Year:  1981        PMID: 7038628      PMCID: PMC327639          DOI: 10.1093/nar/9.24.6763

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN: 0305-1048            Impact factor:   16.971


  23 in total

1.  Export without proteolytic processing of inner and outer membrane proteins encoded by F sex factor tra cistrons in Escherichia coli minicells.

Authors:  M Achtman; P A Manning; C Edelbluth; P Herrlich
Journal:  Proc Natl Acad Sci U S A       Date:  1979-10       Impact factor: 11.205

2.  Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors:  D B Clewell; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1969-04       Impact factor: 11.205

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  The functional organization of the nopaline A. tumefaciens plasmid pTiC58.

Authors:  M Holsters; B Silva; F Van Vliet; C Genetello; M De Block; P Dhaese; A Depicker; D Inzé; G Engler; R Villarroel
Journal:  Plasmid       Date:  1980-03       Impact factor: 3.466

5.  A coupled transcription--translation system derived from Escherichia coli: the use of immobilized deoxyribonuclease to eliminate endogenous DNA.

Authors:  A F Moorman; F Lamie; L A Grivell
Journal:  FEBS Lett       Date:  1976-11-15       Impact factor: 4.124

6.  The use of transposons to introduce well-defined deletions in plasmids: possibilities for in vivo cloning.

Authors:  J Hille; R Schilperoort
Journal:  Plasmid       Date:  1981-07       Impact factor: 3.466

7.  Genetic map of an octopine TI-plasmid.

Authors:  B P Koekman; G Ooms; P M Klapwijk; R A Schilperoort
Journal:  Plasmid       Date:  1979-07       Impact factor: 3.466

8.  Proteins encoded by Agrobacterium tumefaciens Ti plasmid DNA (T-DNA) in crown gall tumors.

Authors:  J C McPherson; E W Nester; M P Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1980-05       Impact factor: 11.205

9.  The nucleoside triphosphate-ribonucleic acid nucleotidyltransferase (EC 2.7.7.6) of Agrobacterium tumefaciens (Smith and Townsend) Conn. Purification and properties of the enzyme from the tumorigenic strain B6806.

Authors:  U C Knopf
Journal:  Biochem J       Date:  1974-12       Impact factor: 3.857

10.  Cell-free synthesis of proteins coding for mobilisation functions of ColE1 and transposition functions of Tn3.

Authors:  J Collins
Journal:  Gene       Date:  1979-05       Impact factor: 3.688

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  1 in total

1.  Mapping of promoter-proximal regions by in vitro transcription of two Agrobacterium tumefaciens Ti-plasmids.

Authors:  G Risuelo; E di Mauro
Journal:  Mol Biol Rep       Date:  1984-07       Impact factor: 2.316

  1 in total

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