Literature DB >> 15049866

Low-cost, frequency-domain, fluorescence lifetime confocal microscopy.

M J Booth1, T Wilson.   

Abstract

We describe the theory and implementation of a frequency-domain fluorescence lifetime confocal microscope using switched diode laser illumination. Standard, communications-type, radio-frequency electronics are used to provide inexpensive modulation references and to perform phase-sensitive detection. This allows the rapid acquisition of fluorescence intensity and lifetime images and their display in real time. We show fluorescence lifetime images of bead objects and fluorescence lifetime images of biological specimens from a single confocal scan.

Mesh：

Year:  2004        PMID： 15049866     DOI： 10.1111/j.0022-2720.2004.01316.x

Source DB:  PubMed          Journal:  J Microsc        ISSN： 0022-2720            Impact factor:   1.758


  12 in total

1.  Polar plot representation for frequency-domain analysis of fluorescence lifetimes.

Authors:  Glen I Redford; Robert M Clegg
Journal:  J Fluoresc       Date:  2005-09       Impact factor: 2.217

2.  High-speed multiparameter photophysical analyses of fluorophore libraries.

Authors:  Kevin M Dean; Lloyd M Davis; Jennifer L Lubbeck; Premashis Manna; Pia Friis; Amy E Palmer; Ralph Jimenez
Journal:  Anal Chem       Date:  2015-04-29       Impact factor: 6.986

3.  Parallel excitation-emission multiplexed fluorescence lifetime confocal microscopy for live cell imaging.

Authors:  Ming Zhao; Yu Li; Leilei Peng
Journal:  Opt Express       Date:  2014-05-05       Impact factor: 3.894

4.  Detection of nucleic acid-protein interactions in plant leaves using fluorescence lifetime imaging microscopy.

Authors:  Laurent Camborde; Alain Jauneau; Christian Brière; Laurent Deslandes; Bernard Dumas; Elodie Gaulin
Journal:  Nat Protoc       Date:  2017-08-24       Impact factor: 13.491

Review 5.  FRAP, FLIM, and FRET: Detection and analysis of cellular dynamics on a molecular scale using fluorescence microscopy.

Authors:  Carla De Los Santos; Ching-Wei Chang; Mary-Ann Mycek; Richard A Cardullo
Journal:  Mol Reprod Dev       Date:  2015-05-25       Impact factor: 2.609

6.  Direct frequency domain fluorescence lifetime imaging using field programmable gate arrays for real time processing.

Authors:  Michael J Serafino; Brian E Applegate; Javier A Jo
Journal:  Rev Sci Instrum       Date:  2020-03-01       Impact factor: 1.523

7.  A multi-functional imaging approach to high-content protein interaction screening.

Authors:  Daniel R Matthews; Gilbert O Fruhwirth; Gregory Weitsman; Leo M Carlin; Enyinnaya Ofo; Melanie Keppler; Paul R Barber; Iain D C Tullis; Borivoj Vojnovic; Tony Ng; Simon M Ameer-Beg
Journal:  PLoS One       Date:  2012-04-10       Impact factor: 3.240

8.  Development of Low-Cost Instrumentation for Single Point Autofluorescence Lifetime Measurements.

Authors:  João Lagarto; Jonathan D Hares; Christopher Dunsby; Paul M W French
Journal:  J Fluoresc       Date:  2017-05-25       Impact factor: 2.217

9.  FRET efficiency measurement in a molecular tension probe with a low-cost frequency-domain fluorescence lifetime imaging microscope.

Authors:  John-Paul Dumas; James Y Jiang; Evan M Gates; Brenton D Hoffman; Mark C Pierce; Nada N Boustany
Journal:  J Biomed Opt       Date:  2019-12       Impact factor: 3.170

10.  Frequency domain photoacoustic and fluorescence microscopy.

Authors:  Gregor Langer; Bianca Buchegger; Jaroslaw Jacak; Thomas A Klar; Thomas Berer
Journal:  Biomed Opt Express       Date:  2016-06-20       Impact factor: 3.732
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