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Literature DB >> 6425355

Validity of an enzyme immunoassay for detection of Neisseria gonorrhoeae antigens.

C J Papasian, W R Bartholomew, D Amsterdam.

Abstract

An enzyme immunoassay (EIA; Gonozyme, Abbott Laboratories) for the antigenic detection of Neisseria gonorrhoeae in endocervical or urethral specimens was evaluated. EIA results were compared with results of conventional culture tests for N. gonorrhoeae. Specimens from 208 males (113 culture positive) and 252 females (72 culture positive) were tested. The sensitivity and specificity of EIA for specimens from males were 97.3 and 95.8%, respectively. The sensitivity and specificity of EIA for specimens from females were 79.2 and 87.2%, respectively.

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Year: 1984 PMID： 6425355 PMCID： PMC271062 DOI： 10.1128/jcm.19.3.347-350.1984

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

13 in total

Validity of an enzyme immunoassay for detection of Neisseria gonorrhoeae antigens.

1. Fluroescent antibody technique for identification of presumptively positive gonococcal cultures.

2. The case for gonococcal serology.

3. Sensitivity and reproducibility of Thayer-Martin culture mddium in diagnosing gonorrhea in women.

4. Observations on the culture diagnosis of gonorrhea in women.

5. Disseminated gonococcal infections caused by Neisseria gonorrhoeae with unique nutritional requirements.

6. Improved transport system for Neisseria gonorrhoeae in clinical specimens.

7. Quantitation of Neisseria gonorrhoeae from women with gonorrhea.

8. Neisseria gonorrhoeae strains inhibited by vancomycin in selective media and correlation with auxotype.

9. Evaluation of the phadebact gonococcus test, a coagglutination procedure for confirmation of Neisseria gonorrhoeae.

10. Detection of Neisseria gonorrhoeae antigen by a solid-phase enzyme immunoassay.

1. Validity and cost-effectiveness of the Gonozyme test in the diagnosis of gonorrhea.

2. Modified enzyme immunoassay for detecting Neisseria gonorrhoeae antigens.

3. Efficacy of an enzyme immunoassay with uncentrifuged first-voided urine for detection of gonorrhea in males.

4. Evaluation of a prototype DNA probe test for the noncultural diagnosis of gonorrhea.

5. Evaluation of a urease-based confirmatory enzyme-linked immunosorbent assay for diagnosis of Neisseria gonorrhoeae.

6. Evaluation of Gonozyme, an enzyme immunoassay for the rapid diagnosis of gonorrhea.

Review 7. Enzyme-linked immunoassays for the detection of microbial antigens and their antibodies.