Literature DB >> 6454891

Ovine prolactin: equilibrium characteristics of the recombinant molecule formed by noncovalent interaction of two fibrinolysin fragments by fluorescence polarization.

M D Jibson, C H Li, C B Glaser.   

Abstract

The equilibrium characteristics of the recombined molecule formed by noncovalent interaction of two fibrinolysin fragments of ovine prolactin (oPRL) have been studied with fluorescence polarization. Fluorescein isothiocyanate (isomer I) was used to label oPRL-(1-53), creating a fluorescent peptide indistinguishable from the unlabeled fragment in the complementation reaction with oPRL-(54-199). The dissociation constant of the recombinant prolactin was 0.144 microM at 30 degrees C, with a free energy of dissociation of 9.50 kcal/mol.

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Year:  1981        PMID： 6454891      PMCID： PMC319451          DOI： 10.1073/pnas.78.5.2830

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  9 in total

1.  APPLICATION OF FLUORESCENCE POLARIZATION TO THE ANTIGEN-ANTIBODY REACTION. THEORY AND EXPERIMENTAL METHOD.

Authors:  W B DANDLIKER; H C SCHAPIRO; J W MEDUSKI; R ALONSO; G A FEIGEN; J R HAMRICK
Journal:  Immunochemistry       Date:  1964-10

2.  Two fragments from fibrinolysin digests of ovine prolactin: characterization and recombination to generate full immunoreactivity.

Authors:  Y Birk; C H Li
Journal:  Proc Natl Acad Sci U S A       Date:  1978-05       Impact factor: 11.205

3.  Studies on pituitary lactogenic hormone. The primary structure of the porcine hormone.

Authors:  C H Li
Journal:  Int J Pept Protein Res       Date:  1976

4.  Rotational Brownian motion and polarization of the fluorescence of solutions.

Authors:  G WEBER
Journal:  Adv Protein Chem       Date:  1953

5.  Reaction of fluorescein-isothiocyanate with proteins and amino acids. II. Preparation of fluorescein-thiohydantoin amino acids and their thin-layer chromatography.

Authors:  H Kawauchi; K Tuzimura; H Maeda; N Ishida
Journal:  J Biochem       Date:  1969-12       Impact factor: 3.387

6.  Studies on pituitary lactogenic hormone. XXX. The primary structure of the sheep hormone.

Authors:  C H Li; J S Dixon; T B Lo; K D Schmidt; Y A Pankov
Journal:  Arch Biochem Biophys       Date:  1970-12       Impact factor: 4.013

7.  Radioimmunoreactivity and receptor-binding activity of the recombined molecule obtained by complementation of two fibrinolysin fragments of ovine prolactin.

Authors:  T M Wong; C H Cheng; C H Li
Journal:  Proc Natl Acad Sci U S A       Date:  1981-01       Impact factor: 11.205

8.  Fluorescence polarization assay of plasmin, plasminogen, and plasminogen activator.

Authors:  K Kinoshita; H Maeda; Y Hinuma
Journal:  Anal Biochem       Date:  1980-05-01       Impact factor: 3.365

9.  Assay of proteolytic enzymes by the fluorescence polarization technique.

Authors:  H Maeda
Journal:  Anal Biochem       Date:  1979-01-01       Impact factor: 3.365
  9 in total

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