Literature DB >> 14927788

The isolation and assay of hypertensin from blood.

L T SKEGGS, J R KAHN, N P SHUMWAY.   

Abstract

A method has been described for isolation and assay of hypertensin from the blood of dogs. An ethanol filtrate of blood drawn from the femoral artery is prepared and concentrated by evaporation. The hypertensin is extracted into n-butanol from which it is adsorbed into alumina, and subsequently eluted with dilute ethanol. The eluate is then evaporated to dryness, dissolved in water, and assayed by intravenous injection into rats.

Entities:  

Keywords:  ANGIOTONIN/determination

Mesh：

Substances：

Year:  1952        PMID： 14927788      PMCID： PMC2212071          DOI： 10.1084/jem.95.3.241

Source DB:  PubMed          Journal:  J Exp Med        ISSN： 0022-1007            Impact factor:   14.307


  2 in total

1.  The isolation of hypertensin from the circulating blood of normal dogs with experimental renal hypertension by dialysis in an artificial kidney.

Authors:  L T SKEGGS; J R KAHN; N P SHUMWAY
Journal:  Circulation       Date:  1951-03       Impact factor: 29.690

2.  The isolation of hypertensin from the circulating blood of dogs by dialysis in an artificial kidney.

Authors:  J R KAHN; L T SKEGGS; N P SHUMWAY
Journal:  Circulation       Date:  1950-09       Impact factor: 29.690
  2 in total
  14 in total

1.  RECENT STUDIES ON I-131-LABELLED ANGIOTENSIN II AND ANALOGUES.

Authors:  R L WOLF; M MENDLOWITZ; J ROBOZ; N E NAFTCHI; S E GITLOW
Journal:  Can Med Assoc J       Date:  1964-01-25       Impact factor: 8.262

2.  NEW PROCEDURES FOR MEASUREMENT OF HUMAN PLASMA ANGIOTENSIN AND RENIN ACTIVITY LEVELS.

Authors:  R BOUCHER; R VEYRAT; J DE CHAMPLAIN; J GENEST
Journal:  Can Med Assoc J       Date:  1964-01-25       Impact factor: 8.262

3.  [Renin and hypertension, physiological or pathological agents?].

Authors:  F GROSS
Journal:  Klin Wochenschr       Date:  1958-08-01

4.  The isolation of a hypertension.

Authors:  W S PEART
Journal:  Biochem J       Date:  1956-03       Impact factor: 3.857

Review 5.  [The renin-angiotensin system: methodology and clinical importance].

Authors:  R Boucher; J Genest
Journal:  Can Med Assoc J       Date:  1970-10-17       Impact factor: 8.262

6.  The purification of hypertensin II.

Authors:  L T SKEGGS; J R KAHN; N P SHUMWAY
Journal:  J Exp Med       Date:  1956-03-01       Impact factor: 14.307

7.  The purification of hypertensin I.

Authors:  L T SKEGGS; W H MARSH; J R KAHN; N P SHUMWAY
Journal:  J Exp Med       Date:  1954-10-01       Impact factor: 14.307

8.  The existence of two forms of hypertensin.

Authors:  L T SKEGGS; W H MARSH; J R KAHN; N P SHUMWAY
Journal:  J Exp Med       Date:  1954-03       Impact factor: 14.307

9.  The preparation, purification, and amino acid sequence of a polypeptide renin substrate.

Authors:  L T SKEGGS; J R KAHN; K LENTZ; N P SHUMWAY
Journal:  J Exp Med       Date:  1957-09-01       Impact factor: 14.307

10.  The synthesis of a tetradecapeptide renin substrate.

Authors:  L T SKEGGS; K E LENTZ; J R KAHN; N P SHUMWAY
Journal:  J Exp Med       Date:  1958-09-01       Impact factor: 14.307
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