Literature DB >> 1482181

Properties of a genetically reconstructed Prevotella ruminicola endoglucanase.

G Maglione1, O Matsushita, J B Russell, D B Wilson.   

Abstract

A pUC19-derived plasmid was constructed that coded for a hybrid cellulase with the Thermomonospora fusca E2 cellulose-binding domain at its C terminus joined to the Prevotella ruminicola 40.5-kDa carboxymethyl cellulase (CMCase). The hybrid enzyme was purified and characterized enzymatically. It bound tightly to cellulose, and its specific activities on carboxymethyl cellulose, amorphous cellulose, and ball-milled cellulose were 1.5, 10, and 8 times that of the 40.5-kDa CMCase, respectively. Furthermore, the modified enzyme gave synergism with an exocellulase in the degradation of filter paper, while the 40.5-kDa CMCase did not.

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Year:  1992        PMID： 1482181      PMCID： PMC183149          DOI： 10.1128/aem.58.11.3593-3597.1992

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  19 in total

1.  THE CELLULOLYTIC ACTIVITY OF PURE STRAINS OF BACTERIA FROM THE RUMEN OF CATTLE.

Authors:  G HALLIWELL; M P BRYANT
Journal:  J Gen Microbiol       Date:  1963-09

Review 2.  Potential opportunities and problems for genetically altered rumen microorganisms.

Authors:  J B Russell; D B Wilson
Journal:  J Nutr       Date:  1988-02       Impact factor: 4.798

3.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Purification and characterization of an exo-beta-1,4-glucanase from Ruminococcus flavefaciens FD-1.

Authors:  R M Gardner; K C Doerner; B A White
Journal:  J Bacteriol       Date:  1987-10       Impact factor: 3.490

6.  Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

Authors:  C Yanisch-Perron; J Vieira; J Messing
Journal:  Gene       Date:  1985       Impact factor: 3.688

7.  A Bacteroides ruminicola 1,4-beta-D-endoglucanase is encoded in two reading frames.

Authors:  O Matsushita; J B Russell; D B Wilson
Journal:  J Bacteriol       Date:  1991-11       Impact factor: 3.490

8.  Precise excision of the cellulose binding domains from two Cellulomonas fimi cellulases by a homologous protease and the effect on catalysis.

Authors:  N R Gilkes; R A Warren; R C Miller; D G Kilburn
Journal:  J Biol Chem       Date:  1988-07-25       Impact factor: 5.157

9.  Effect of pH on the efficiency of growth by pure cultures of rumen bacteria in continuous culture.

Authors:  J B Russell; D B Dombrowski
Journal:  Appl Environ Microbiol       Date:  1980-03       Impact factor: 4.792

10.  Cellulolytic activity of the rumen bacterium Bacteroides succinogenes.

Authors:  D Groleau; C W Forsberg
Journal:  Can J Microbiol       Date:  1981-05       Impact factor: 2.419
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  12 in total

1.  Structure-function analysis of the bacterial expansin EXLX1.

Authors:  Nikolaos Georgelis; Akira Tabuchi; Nikolas Nikolaidis; Daniel J Cosgrove
Journal:  J Biol Chem       Date:  2011-03-24       Impact factor: 5.157

2.  Pseudomonas cellulose-binding domains mediate their effects by increasing enzyme substrate proximity.

Authors:  D N Bolam; A Ciruela; S McQueen-Mason; P Simpson; M P Williamson; J E Rixon; A Boraston; G P Hazlewood; H J Gilbert
Journal:  Biochem J       Date:  1998-05-01       Impact factor: 3.857

3.  Kinetics of Cellulose Digestion by Fibrobacter succinogenes S85.

Authors:  G Maglione; J B Russell; D B Wilson
Journal:  Appl Environ Microbiol       Date:  1997-02       Impact factor: 4.792

4.  Xylanase B from Neocallimastix patriciarum contains a non-catalytic 455-residue linker sequence comprised of 57 repeats of an octapeptide.

Authors:  G W Black; G P Hazlewood; G P Xue; C G Orpin; H J Gilbert
Journal:  Biochem J       Date:  1994-04-15       Impact factor: 3.857

5.  Biological pretreatment with two bacterial strains for enzymatic hydrolysis of office paper.

Authors:  Masahiro Kurakake; Nozomi Ide; Toshiaki Komaki
Journal:  Curr Microbiol       Date:  2007-05-08       Impact factor: 2.188

6.  The cellular location of Prevotella ruminicola beta-1,4-D-endoglucanase and its occurrence in other strains of ruminal bacteria.

Authors:  R G Gardner; J E Wells; J B Russell; D B Wilson
Journal:  Appl Environ Microbiol       Date:  1995-09       Impact factor: 4.792

7.  Use of a modified Bacteroides-Prevotella shuttle vector to transfer a reconstructed beta-1,4-D-endoglucanase gene into Bacteroides uniformis and Prevotella ruminicola B(1)4.

Authors:  R G Gardner; J B Russell; D B Wilson; G R Wang; N B Shoemaker
Journal:  Appl Environ Microbiol       Date:  1996-01       Impact factor: 4.792

Review 8.  Carbohydrate-binding modules: fine-tuning polysaccharide recognition.

Authors:  Alisdair B Boraston; David N Bolam; Harry J Gilbert; Gideon J Davies
Journal:  Biochem J       Date:  2004-09-15       Impact factor: 3.857

9.  The non-catalytic cellulose-binding domain of a novel cellulase from Pseudomonas fluorescens subsp. cellulosa is important for the efficient hydrolysis of Avicel.

Authors:  J Hall; G W Black; L M Ferreira; S J Millward-Sadler; B R Ali; G P Hazlewood; H J Gilbert
Journal:  Biochem J       Date:  1995-08-01       Impact factor: 3.857

10.  Influence of a mannan binding family 32 carbohydrate binding module on the activity of the appended mannanase.

Authors:  Kimiya Mizutani; Vânia O Fernandes; Shuichi Karita; Ana S Luís; Makiko Sakka; Tetsuya Kimura; Adam Jackson; Xiaoyang Zhang; Carlos M G A Fontes; Harry J Gilbert; Kazuo Sakka
Journal:  Appl Environ Microbiol       Date:  2012-05-04       Impact factor: 4.792
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