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Literature DB >> 14755157

A selective intra-HN(CA)CO experiment for the backbone assignment of deuterated proteins.

Abstract

A modified version of the backbone TROSY based 3D HN(CA)CO experiment is presented that provides uniquely intraresidue correlation shift information, by efficiently suppressing the sequential connectivity data. The resulting reduction in the number of peaks decreases overlap and removes ambiguities in situations where in the conventional HN(CA)CO i and i - 1 correlations are overlapped or are comparable in intensity. The sequential residue connection is obtained in combination with a 3D HNCO. MQ- and SQ-implementations of the experiment were tested on a deuterated protein with a correlation time of 28 ns ( approximately 60 kDa).

Entities: Disease Gene

Mesh：

Substances：
Proteins
Deuterium

Year: 2004 PMID： 14755157 DOI： 10.1023/B:JNMR.0000013829.17620.39

Source DB: PubMed Journal: J Biomol NMR ISSN： 0925-2738 Impact factor: 2.835

17 in total

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Journal: J Am Chem Soc Date: 2002-09-18 Impact factor: 15.419

4. Use of selective C alpha pulses for improvement of HN(CA)CO-D and HN(COCA)NH-D experiments.

Authors: H Matsuo; E Kupce; H Li; G Wagner
Journal: J Magn Reson B Date: 1996-05

5. Short selective pulses for biochemical applications.

Authors: E Kupce; J Boyd; I D Campbell
Journal: J Magn Reson B Date: 1995-03

6. [13C]-constant-time [15N,1H]-TROSY-HNCA for sequential assignments of large proteins.

Authors: M Salzmann; K Pervushin; G Wider; H Senn; K Wüthrich
Journal: J Biomol NMR Date: 1999-05 Impact factor: 2.835

7. Transverse relaxation-optimized NMR spectroscopy with the outer membrane protein OmpX in dihexanoyl phosphatidylcholine micelles.

Authors: C Fernández; K Adeishvili; K Wüthrich
Journal: Proc Natl Acad Sci U S A Date: 2001-02-20 Impact factor: 11.205

8. Improved sensitivity and coherence selection for [15N,1H]-TROSY elements in triple resonance experiments.

Authors: M Salzmann; G Wider; K Pervushin; K Wüthrich
Journal: J Biomol NMR Date: 1999-10 Impact factor: 2.835

9. Intraresidual HNCA: an experiment for correlating only intraresidual backbone resonances.

Authors: Perttu Permi
Journal: J Biomol NMR Date: 2002-07 Impact factor: 2.835

10. A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin.

Authors: M Ikura; L E Kay; A Bax
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4 in total