Literature DB >> 14745172

Proteomic identification of alpha-amylase isoforms encoded by RAmy3B/3C from germinating rice seeds.

Yohei Nanjo1, Satoru Asatsuma, Kimiko Itoh, Hidetaka Hori, Toshiaki Mitsui.   

Abstract

We isolated and identified 10 alpha-amylase isoforms by using beta-cyclodextrin Sepharose affinity column chromatography and two-dimensional polyacrylamide gel electrophoresis from germinating rice (Oryza sativa L.) seeds. Immunoblots with anti-alpha-amylase I-1 and II-4 antibodies indicated that 8 isoforms in 10 are distinguishable from alpha-amylase I-1 and II-4. Peptide mass fingerprinting analysis showed that there exist novel isoforms encoded by RAmy3B and RAmy3C genes. The optimum temperature for enzyme reaction of the RAmy3B and RAmy3C coding isoforms resembled that of alpha-amylase isoform II-4 (RAmy3D). Furthermore, complex protein polymorphism resulted from a single alpha-amylase gene was found to occur not only in RAmy3D, but also in RAmy3B.

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Year:  2004        PMID： 14745172     DOI： 10.1271/bbb.68.112

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  7 in total

1.  The rice alpha-amylase glycoprotein is targeted from the Golgi apparatus through the secretory pathway to the plastids.

Authors:  Aya Kitajima; Satoru Asatsuma; Hisao Okada; Yuki Hamada; Kentaro Kaneko; Yohei Nanjo; Yasushi Kawagoe; Kiminori Toyooka; Ken Matsuoka; Masaki Takeuchi; Akihiko Nakano; Toshiaki Mitsui
Journal:  Plant Cell       Date:  2009-09-18       Impact factor: 11.277

2.  Senescence-Specific Expression of RAmy1A Accelerates Non-structural Carbohydrate Remobilization and Grain Filling in Rice (Oryza sativa L.).

Authors:  Ning Ouyang; Xuewu Sun; Yanning Tan; Zhizhong Sun; Dong Yu; Hai Liu; Citao Liu; Ling Liu; Lu Jin; Bingran Zhao; Dingyang Yuan; Meijuan Duan
Journal:  Front Plant Sci       Date:  2021-04-27       Impact factor: 5.753

3.  Characters related to higher starch accumulation in cassava storage roots.

Authors:  You-Zhi Li; Jian-Yu Zhao; San-Min Wu; Xian-Wei Fan; Xing-Lu Luo; Bao-Shan Chen
Journal:  Sci Rep       Date:  2016-02-19       Impact factor: 4.379

4.  Proteomic and Glycomic Characterization of Rice Chalky Grains Produced Under Moderate and High-temperature Conditions in Field System.

Authors:  Kentaro Kaneko; Maiko Sasaki; Nanako Kuribayashi; Hiromu Suzuki; Yukiko Sasuga; Takeshi Shiraya; Takuya Inomata; Kimiko Itoh; Marouane Baslam; Toshiaki Mitsui
Journal:  Rice (N Y)       Date:  2016-05-31       Impact factor: 4.783

5.  High Temperature-Induced Expression of Rice α-Amylases in Developing Endosperm Produces Chalky Grains.

Authors:  Masaru Nakata; Yosuke Fukamatsu; Tomomi Miyashita; Makoto Hakata; Rieko Kimura; Yuriko Nakata; Masaharu Kuroda; Takeshi Yamaguchi; Hiromoto Yamakawa
Journal:  Front Plant Sci       Date:  2017-12-06       Impact factor: 5.753

6.  Enzyme activities and gene expression of starch metabolism provide insights into grape berry development.

Authors:  Xudong Zhu; Chaobo Zhang; Weimin Wu; Xiaopeng Li; Chuan Zhang; Jinggui Fang
Journal:  Hortic Res       Date:  2017-05-10       Impact factor: 6.793

7.  Overexpression of a wheat α-amylase type 2 impact on starch metabolism and abscisic acid sensitivity during grain germination.

Authors:  Qin Zhang; Jenifer Pritchard; Jos Mieog; Keren Byrne; Michelle L Colgrave; Ji-Rui Wang; Jean-Philippe F Ral
Journal:  Plant J       Date:  2021-08-20       Impact factor: 7.091
  7 in total

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