Literature DB >> 1471985

The control of cellular shape and motility. Mg2+ and tropomyosin regulate the formation and the dissociation of microfilament bundles.

E Grazi1, P Cuneo, A Cataldi.   

Abstract

At pH 7.14 and 37 degrees C, in 7.2% (w/v) poly(ethylene glycol) 6000, tropomyosin-regulated actin filaments are converted into filament bundles by increasing the free Mg2+ concentration to 1.7-2.0 mM. When free Mg2+ concentration is decreased below 1.7 mM, bundles dissociate back into tropomyosin-regulated actin filaments. Pure actin filaments are insensitive to this mechanism of control and are found as filament bundles in all the range of free Mg2+ concentrations tested (1.37-2.2 mM). The mechanism of regulation described above is likely to operate in the cell, where the concentration of free Mg2+ is linked to the energy charge of the adenine nucleotide system.

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Year:  1992        PMID： 1471985      PMCID： PMC1131946          DOI： 10.1042/bj2880727

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  38 in total

1.  Reorganization of actin in platelets stimulated by thrombin as measured by the DNase I inhibition assay.

Authors:  L Carlsson; F Markey; I Blikstad; T Persson; U Lindberg
Journal:  Proc Natl Acad Sci U S A       Date:  1979-12       Impact factor: 11.205

2.  The control of cellular motility and the role of gelsolin.

Authors:  E Grazi; E Magri; P Cuneo; A Cataldi
Journal:  FEBS Lett       Date:  1991-12-16       Impact factor: 4.124

3.  Evidence that unphosphorylated smooth muscle myosin supports smooth muscle contraction.

Authors:  E Grazi; G Trombetta
Journal:  Biochem Biophys Res Commun       Date:  1991-08-15       Impact factor: 3.575

Review 4.  Nonmuscle actin-binding proteins.

Authors:  T P Stossel; C Chaponnier; R M Ezzell; J H Hartwig; P A Janmey; D J Kwiatkowski; S E Lind; D B Smith; F S Southwick; H L Yin
Journal:  Annu Rev Cell Biol       Date:  1985

5.  Increased uniformity in the response of the coomassie blue G protein assay to different proteins.

Authors:  C M Stoscheck
Journal:  Anal Biochem       Date:  1990-01       Impact factor: 3.365

6.  The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.

Authors:  J A Spudich; S Watt
Journal:  J Biol Chem       Date:  1971-08-10       Impact factor: 5.157

7.  Effect of pH and metal ion concentration on the equilibrium hydrolysis of adenosine triphosphate to adenosine diphosphate.

Authors:  R A Alberty
Journal:  J Biol Chem       Date:  1968-04-10       Impact factor: 5.157

Review 8.  Actin and actin-binding proteins. A critical evaluation of mechanisms and functions.

Authors:  T D Pollard; J A Cooper
Journal:  Annu Rev Biochem       Date:  1986       Impact factor: 23.643

9.  Monitoring cytosolic free magnesium in cultured chicken heart cells by use of the fluorescent indicator Furaptra.

Authors:  E Murphy; C C Freudenrich; L A Levy; R E London; M Lieberman
Journal:  Proc Natl Acad Sci U S A       Date:  1989-04       Impact factor: 11.205

10.  Measurement and control of intracellular magnesium ion concentration in guinea pig and ferret ventricular myocardium.

Authors:  C H Fry
Journal:  Magnesium       Date:  1986
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  1 in total

1.  The bundling of actin with polyethylene glycol 8000 in the presence and absence of gelsolin.

Authors:  J Goverman; L A Schick; J Newman
Journal:  Biophys J       Date:  1996-09       Impact factor: 4.033
  1 in total

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