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Literature DB >> 14715792

Impact of DNA polymerases and their buffer systems on quantitative real-time PCR.

Petra Wolffs¹, Halfdan Grage, Oskar Hagberg, Peter Rådström.

Abstract

An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that the choice of both DNA polymerase and the buffer system affected the amplification efficiency as well as the detection window. The analytical repeatability of the data for different systems changed clearly, leading us to conclude that basing quantitative measurements on single-data-set standard curves can lead to significant errors.

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Year: 2004 PMID： 14715792 PMCID： PMC321720 DOI： 10.1128/JCM.42.1.408-411.2004

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

9 in total

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Authors: Nichole E Brinkman; Richard A Haugland; Larry J Wymer; Muruleedhara Byappanahalli; Richard L Whitman; Stephen J Vesper
Journal: Appl Environ Microbiol Date: 2003-03 Impact factor: 4.792

3. Comparison of real-time PCR with SYBR Green I or 5'-nuclease assays and dot-blot hybridization with rDNA-targeted oligonucleotide probes in quantification of selected faecal bacteria.

Authors: Erja Malinen; Anna Kassinen; Teemu Rinttilä; Airi Palva
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4. Quantification of murine IFN-gamma mRNA and protein expression: impact of real-time kinetic RT-PCR using SYBR green I dye.

Authors: J Hein; U Schellenberg; G Bein; H Hackstein
Journal: Scand J Immunol Date: 2001-09 Impact factor: 3.487

5. Proviral load determination of different feline immunodeficiency virus isolates using real-time polymerase chain reaction: influence of mismatches on quantification.

Authors: D Klein; P Janda; R Steinborn; M Müller; B Salmons; W H Günzburg
Journal: Electrophoresis Date: 1999-02 Impact factor: 3.535

6. Detection and quantification of Escherichia coli O157:H7 in environmental samples by real-time PCR.

Authors: A M Ibekwe; C M Grieve
Journal: J Appl Microbiol Date: 2003 Impact factor: 3.772

7. Application of the 5'-nuclease PCR assay in evaluation and development of methods for quantitative detection of Campylobacter jejuni.

Authors: H K Nogva; A Bergh; A Holck; K Rudi
Journal: Appl Environ Microbiol Date: 2000-09 Impact factor: 4.792

8. Modeling of 5' nuclease real-time responses for optimization of a high-throughput enrichment PCR procedure for Salmonella enterica.

Authors: Rickard Knutsson; Charlotta Löfström; Halfdan Grage; Jeffrey Hoorfar; Peter Rådström
Journal: J Clin Microbiol Date: 2002-01 Impact factor: 5.948

9. Detection of pathogenic Yersinia enterocolitica in enrichment media and pork by a multiplex PCR: a study of sample preparation and PCR-inhibitory components.

Authors: P G Lantz; R Knutsson; Y Blixt; W A Al Soud; E Borch; P Rådström
Journal: Int J Food Microbiol Date: 1998-12-08 Impact factor: 5.277

9 in total

15 in total

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Authors: Gerhard F Weldhagen
Journal: Antimicrob Agents Chemother Date: 2004-10 Impact factor: 5.191

Review 2. Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction.

Authors: Stephen A Bustin; Tania Nolan
Journal: J Biomol Tech Date: 2004-09

3. Direct quantitation and detection of salmonellae in biological samples without enrichment, using two-step filtration and real-time PCR.

Authors: Petra F G Wolffs; Kari Glencross; Romain Thibaudeau; Mansel W Griffiths
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Review 4. Enumeration of salmonella bacteria in food and feed samples by real-time PCR for quantitative microbial risk assessment.

Authors: Burkhard Malorny; Charlotta Löfström; Martin Wagner; Nadine Krämer; Jeffrey Hoorfar
Journal: Appl Environ Microbiol Date: 2007-12-28 Impact factor: 4.792

5. Rapid quantification of Yersinia enterocolitica in pork samples by a novel sample preparation method, flotation, prior to real-time PCR.

Authors: Petra Wolffs; Rickard Knutsson; Börje Norling; Peter Rådström
Journal: J Clin Microbiol Date: 2004-03 Impact factor: 5.948

6. Production of random DNA oligomers for scalable DNA computing.

Authors: Sixue S L Wang; John J X Johnson; Bradley S T Hughes; Dundar A O Karabay; Karson D W Bader; Allen Austin; Alan Austin; Aisha Habib; Husnia Hatef; Megha Joshi; Lawrence Nguyen; Allen P Mills
Journal: Biotechnol J Date: 2009-01 Impact factor: 4.677

7. Comparative analysis of four methods to extract DNA from paraffin-embedded tissues: effect on downstream molecular applications.

Authors: Cornelis Jj Huijsmans; Jan Damen; Johannes C van der Linden; Paul Hm Savelkoul; Mirjam Ha Hermans
Journal: BMC Res Notes Date: 2010-09-14