Literature DB >> 14704363

Improved full-length cDNA production based on RNA tagging by T4 DNA ligase.

Christian Clepet1, Isabelle Le Clainche, Michel Caboche.   

Abstract

Second-strand cDNA priming is a central problem for full-length characterization of transcripts. A new strategy using bacteriophage T4 DNA ligase and partially degenerate adapters is proposed for grafting a sequence tag to the end of polyribonucleotides. Based on this RNA tagging system and previously described protocols, a new method for full-length cDNA production has been implemented. Validation of the method is shown in Arabidopsis thaliana by the construction of a full-length cDNA library and the analysis of 154 clones and by 5'-RACE-PCR run on a documented experimental system.

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Year:  2004        PMID： 14704363      PMCID： PMC373303          DOI： 10.1093/nar/gng158

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  20 in total

1.  Joining RNA molecules with T4 DNA ligase.

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Journal:  Methods Mol Biol       Date:  1999

2.  Directional cDNA library construction assisted by the in vitro recombination reaction.

Authors:  O Ohara; G Temple
Journal:  Nucleic Acids Res       Date:  2001-02-15       Impact factor: 16.971

3.  FLAGdb/FST: a database of mapped flanking insertion sites (FSTs) of Arabidopsis thaliana T-DNA transformants.

Authors:  F Samson; V Brunaud; S Balzergue; B Dubreucq; L Lepiniec; G Pelletier; M Caboche; A Lecharny
Journal:  Nucleic Acids Res       Date:  2002-01-01       Impact factor: 16.971

4.  A novel cDNA/PCR strategy for efficient cloning of small amounts of undefined RNA.

Authors:  A Akowitz; L Manuelidis
Journal:  Gene       Date:  1989-09-30       Impact factor: 3.688

5.  Detection of the 5'-cap structure of messenger RNAs with the use of the cap-jumping approach.

Authors:  V A Efimov; O G Chakhmakhcheva; J Archdeacon; J M Fernandez; O N Fedorkin; Y L Dorokhov; J G Atabekov
Journal:  Nucleic Acids Res       Date:  2001-11-15       Impact factor: 16.971

6.  Synthesis of full-length cDNA using DNA-capped mRNA.

Authors:  S Sekine; S Kato
Journal:  Nucleic Acids Symp Ser       Date:  1993

7.  A highly sensitive method for mapping the 5' termini of mRNAs.

Authors:  M Fromont-Racine; E Bertrand; R Pictet; T Grange
Journal:  Nucleic Acids Res       Date:  1993-04-11       Impact factor: 16.971

8.  Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.

Authors:  K Maruyama; S Sugano
Journal:  Gene       Date:  1994-01-28       Impact factor: 3.688

9.  Analysis of the genome sequence of the flowering plant Arabidopsis thaliana.

Authors: 
Journal:  Nature       Date:  2000-12-14       Impact factor: 49.962

10.  Oligodeoxyribonucleotide ligation to single-stranded cDNAs: a new tool for cloning 5' ends of mRNAs and for constructing cDNA libraries by in vitro amplification.

Authors:  J B Edwards; J Delort; J Mallet
Journal:  Nucleic Acids Res       Date:  1991-10-11       Impact factor: 16.971
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  10 in total

1.  Evaluation of vector-primed cDNA library production from microgram quantities of total RNA.

Authors:  Jonathan Kuo; Jason Inman; Michael Brownstein; Ted B Usdin
Journal:  Nucleic Acids Res       Date:  2004-12-15       Impact factor: 16.971

2.  Direct comparison of nick-joining activity of the nucleic acid ligases from bacteriophage T4.

Authors:  Desmond R Bullard; Richard P Bowater
Journal:  Biochem J       Date:  2006-08-15       Impact factor: 3.857

3.  Regulation and function of Escherichia coli sugar efflux transporter A (SetA) during glucose-phosphate stress.

Authors:  Yan Sun; Carin K Vanderpool
Journal:  J Bacteriol       Date:  2010-10-22       Impact factor: 3.490

4.  Mapping genomic hotspots of DNA damage by a single-strand-DNA-compatible and strand-specific ChIP-seq method.

Authors:  Zhi-Xiong Zhou; Mei-Jun Zhang; Xu Peng; Yuko Takayama; Xing-Ya Xu; Ling-Zhi Huang; Li-Lin Du
Journal:  Genome Res       Date:  2012-12-17       Impact factor: 9.043

5.  RNA captor: a tool for RNA characterization.

Authors:  Christian Clepet
Journal:  PLoS One       Date:  2011-04-13       Impact factor: 3.240

6.  The phage T4 DNA ligase mediates bacterial chromosome DSBs repair as single component non-homologous end joining.

Authors:  Tianyuan Su; Fapeng Liu; Yizhao Chang; Qi Guo; Junshu Wang; Qian Wang; Qingsheng Qi
Journal:  Synth Syst Biotechnol       Date:  2019-05-16

7.  A new set of ESTs and cDNA clones from full-length and normalized libraries for gene discovery and functional characterization in citrus.

Authors:  M Carmen Marques; Hugo Alonso-Cantabrana; Javier Forment; Raquel Arribas; Santiago Alamar; Vicente Conejero; Miguel A Perez-Amador
Journal:  BMC Genomics       Date:  2009-09-11       Impact factor: 3.969

8.  Gene discovery and transcript analyses in the corn smut pathogen Ustilago maydis: expressed sequence tag and genome sequence comparison.

Authors:  Eric C H Ho; Matt J Cahill; Barry J Saville
Journal:  BMC Genomics       Date:  2007-09-24       Impact factor: 3.969

9.  Cloning and plant-based production of antibody MC10E7 for a lateral flow immunoassay to detect [4-arginine]microcystin in freshwater.

Authors:  Stanislav Melnik; Anna-Cathrine Neumann; Ryan Karongo; Sebastian Dirndorfer; Martin Stübler; Verena Ibl; Reinhard Niessner; Dietmar Knopp; Eva Stoger
Journal:  Plant Biotechnol J       Date:  2017-06-05       Impact factor: 13.263

10.  Detecting DNA double-stranded breaks in mammalian genomes by linear amplification-mediated high-throughput genome-wide translocation sequencing.

Authors:  Jiazhi Hu; Robin M Meyers; Junchao Dong; Rohit A Panchakshari; Frederick W Alt; Richard L Frock
Journal:  Nat Protoc       Date:  2016-03-31       Impact factor: 13.491
  10 in total

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