| Literature DB >> 8247743 |
Abstract
We have developed a novel method for synthesizing a full-length cDNA based on the addition of DNA-RNA chimeric linker to the CAP site of mRNA. The DNA-capped mRNA was used as a template for cDNA synthesis with a dT-tailed vector primer. The model experiment using rabbit globin mRNA showed that more than 80% of the clones encoding alpha- and beta-globin contains a full-length cDNAs starting from the CAP site.Entities:
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Year: 1993 PMID： 8247743
Source DB: PubMed Journal: Nucleic Acids Symp Ser ISSN： 0261-3166