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Literature DB >> 146879

Cytoplasmic inheritance of rutamycin resistance in mouse fibroblasts.

Abstract

Mouse fibroblasts resistant to the drug rutamycin were isolated by selectively introducing BrdUrd into the mitochondrial genome of a line of mouse fibroblasts (clone 1 D) lacking a cytoplasmic thymidine kinase enzyme. The ATPase (ATP phosphohydrolase; EC 3.6.1.3) activity of mitochondria isolated from these cells was resistant to rutamycin. The rutamycin-resistant mutants were enucleated with cytochalasin B and fused with mouse A 9 cells resistant to 8-azaguanine and sensitive to rutamycin. Cytoplasmic hybrids, or cybrids, were selected as cells resistant to rutamycin and 8-azaguanine, and appeared at a high frequency. Other fusions between rutamycin-resistant nucleated cells and A 9 produced colonies at a much lower frequency. Finally, fusions between enucleated clone 1 D cells and A 9 cells produced no rutamycin-resistant colonies. These results indicate that rutamycin resistance is a cytoplasmically inherited characteristic in this cell line.

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Year: 1978 PMID： 146879 PMCID： PMC411240 DOI： 10.1073/pnas.75.1.324

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

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