OBJECTIVE: The goal of this study was to determine whether expression of matrix metalloproteinases (MMP) in tympanic membranes (TM) is stimulated by host tumor necrosis factor-alpha (TNF-alpha) and bacterial toxin (lipopolysaccharide, LPS), which may play a role in the pathogenesis of cholesteatoma. STUDY DESIGN: Gerbil TMs (n = 48) were cultured in the presence of either TNF-alpha or LPS. Quantitative analysis of TNF-alpha and MMPs was performed on both the homogenized TMs and the culture media supernatant. RESULTS: Levels of TNF-alpha and MMPs did not increase significantly in TMs with exposure to inflammatory mediators. TNF-alpha levels in supernatant were elevated (P < 0.007) with more concentrated LPS exposure. Supernatant levels of MMP2 were increased (P < 0.007) at most concentrations of LPS and TNF-alpha. CONCLUSION: Cultured TMs expressed MMPs in response to bacterial and host inflammatory mediators. These findings support a link between inflammation and the secretion of potentially destructive MMPs in the TM, which may contribute to the pathogenesis of cholesteatoma.
OBJECTIVE: The goal of this study was to determine whether expression of matrix metalloproteinases (MMP) in tympanic membranes (TM) is stimulated by host tumor necrosis factor-alpha (TNF-alpha) and bacterial toxin (lipopolysaccharide, LPS), which may play a role in the pathogenesis of cholesteatoma. STUDY DESIGN: Gerbil TMs (n = 48) were cultured in the presence of either TNF-alpha or LPS. Quantitative analysis of TNF-alpha and MMPs was performed on both the homogenized TMs and the culture media supernatant. RESULTS: Levels of TNF-alpha and MMPs did not increase significantly in TMs with exposure to inflammatory mediators. TNF-alpha levels in supernatant were elevated (P < 0.007) with more concentrated LPS exposure. Supernatant levels of MMP2 were increased (P < 0.007) at most concentrations of LPS and TNF-alpha. CONCLUSION: Cultured TMs expressed MMPs in response to bacterial and host inflammatory mediators. These findings support a link between inflammation and the secretion of potentially destructive MMPs in the TM, which may contribute to the pathogenesis of cholesteatoma.
Authors: Nathan B Sautter; Katherine L Delaney; Frances A Hausman; Dennis R Trune Journal: Int J Pediatr Otorhinolaryngol Date: 2011-09-01 Impact factor: 1.675
Authors: Steven K Juhn; Min-Kyo Jung; Mark D Hoffman; Brian R Drew; Diego A Preciado; Nicholas J Sausen; Timothy T K Jung; Bo Hyung Kim; Sang-Yoo Park; Jizhen Lin; Frank G Ondrey; David R Mains; Tina Huang Journal: Clin Exp Otorhinolaryngol Date: 2008-09-30 Impact factor: 3.372