Risk factors for antibiotic resistance in Campylobacter spp. isolated from raw poultry meat in Switzerland.

BACKGROUND: The world-wide increase of foodborne infections with antibiotic resistant pathogens is of growing concern and is designated by the World Health Organization as an emerging public health problem. Thermophilic Campylobacter have been recognised as a major cause of foodborne bacterial gastrointestinal human infections in Switzerland and in many other countries throughout the world. Poultry meat is the most common source for foodborne cases caused by Campylobacter. Because all classes of antibiotics recommended for treatment of human campylobacteriosis are also used in veterinary medicine, in view of food safety, the resistance status of Campylobacter isolated from poultry meat is of special interest.
METHODS: Raw poultry meat samples were collected throughout Switzerland and Liechtenstein at retail level and examined for Campylobacter spp. One strain from each Campylobacter-positive sample was selected for susceptibility testing with the disc diffusion and the E-test method. Risk factors associated with resistance to the tested antibiotics were analysed by multiple logistic regression.
RESULTS: In total, 91 Campylobacter spp. strains were isolated from 415 raw poultry meat samples. Fifty-one strains (59%) were sensitive to all tested antibiotics. Nineteen strains (22%) were resistant to a single, nine strains to two antibiotics, and eight strains showed at least three antibiotic resistances. Resistance was observed most frequently to ciprofloxacin (28.7%), tetracycline (12.6%), sulphonamide (11.8%), and ampicillin (10.3%). One multiple resistant strain exhibited resistance to five antibiotics including ciprofloxacin, tetracycline, and erythromycin. These are the most important antibiotics for treatment of human campylobacteriosis. A significant risk factor associated with multiple resistance in Campylobacter was foreign meat production compared to Swiss meat production (odds ratio = 5.7).
CONCLUSION: Compared to the situation in other countries, the data of this study show a favourable resistance situation for Campylobacter strains isolated from raw poultry meat produced in Switzerland. Nevertheless, the prevalence of 19% ciprofloxacin resistant strains is of concern and has to be monitored. "Foreign production vs. Swiss production" was a significant risk factor for multiple resistance in the logistic regression model. Therefore, an adequate resistance-monitoring programme should include meat produced in Switzerland as well as imported meat samples.

Background

The importance of Campylobacter jejuni and Campylobacter coli as foodborne pathogens has steadily increased during the last years. With an incidence of 92 reported cases per 100'000 inhabitants, Campylobacter spp. are the leading cause of bacterial zoonosis in Switzerland [1]. Inadequately cooked meat, particularly poultry, unpasteurised milk and contaminated drinking water are the most common sources for epidemic and sporadic foodborne cases [2,3]. Due to the often self-limiting diarrhoea in humans, antibiotic treatment is normally not required. Nevertheless, antibiotic treatment is indicated for severe and prolonged enteritis, septicaemia, and for persons at risk such as very young or immuno-compromised patients. Besides erythromycin, which is the antibiotic of choice, fluoroquinolones and tetracycline are commonly used [4]. Particularly fluoroquinolones are first-line drugs for empiric therapy of acute diarrhoea, as they are effective against most major pathogens causing bacterial enteritis [5,6]. Therefore, most cases of campylobacteriosis receiving antibiotic treatment will initially be treated with fluoroquinolones. Worrying is the fact, that in recent years a rapidly increasing proportion of Campylobacter isolates from humans and animals all over the world were found to be resistant to fluoroquinolones [7-11]. This coincided with initiation of the use of the fluoroquinolones to food animals in many countries [12-15].

The objectives of this study were i) to determine the prevalence of antibiotic resistant Campylobacter strains in raw poultry meat samples at retail level, and ii) to identify possible risk factors associated.

Methods

For sampling, 122 retail stores throughout Switzerland and Liechtenstein were randomly selected. From March until July 2002, food safety inspectors of the cantonal laboratories collected 415 samples of raw poultry meat (whole chickens as well as parts such as cutlet, meat cut into strips, legs, drumsticks, wings, and breast) from selected stores. Additionally, information about housing type of the poultry (as labelled), the country of origin (as labelled), refrigeration (chilled, frozen), and packing of the samples was recorded.

Of each sample, 10 g was inoculated into 100 ml selective enrichment broth (Brucella broth (211088, Becton Dickinson, Sparks, USA) with Campylobacter growth and selective supplement (SR084E, SR069E, Oxoid, Basingstoke, Hampshire, UK)) and incubated at 42°C for 24 h under microaerobic conditions provided by commercial gas packs (CampyGen from Oxoid). After enrichment the samples were streaked on selective agar media (Brucella agar (211086 Becton Dickinson) with 6% horse blood (SR0048C, Oxoid)) as well as Butzler Campylobacter selective supplement (SR085E, Oxoid) and incubated at 42°C for another 24 h under microaerobic conditions. Translucent white, moist and glistening colonies were picked and taken for further identification. Identification of Campylobacter strains was performed using the following standard tests: gram-negative stain, characteristic motion, catalase- and oxidase reactions, aerobic growth. One strain from each Campylobacter-positive sample was selected for susceptibility testing.

The disc diffusion method was performed as recommended by the National Committee for Clinical Laboratory Standards [16]. The following antibiotic impregnated discs (bioMérieux SA, France) were used: erythromycin (15 μg), ciprofloxacin (5 μg), tetracycline (30 μg), gentamicin (10 μg), streptomycin (10 μg), ampicillin (10 μg), amoxicillin (25 μg) and sulphonamide (20 μg). Three to five isolated colonies of the same morphological type were selected from the agar plate culture and transferred into trypticase soy broth (211768, Becton Dickinson). After incubation at 42°C for 24 h under microaerobic conditions a sterile cotton swab was dipped into the suspension and streaked on the entire surface of a Mueller-Hinton agar (CM 337, Oxoid) with 5% sheep blood. Four antibiotic discs were placed on each plate and after 48 h of microaerobic incubation at 42°C the diameter of the inhibition zone was measured with calipers. E. coli ATCC 25922 and S. aureus ATCC 25923 were used as reference strains. Zones of growth inhibition were evaluated according to the NCCLS standards.

The E-test (AB Biodisk, Solna, Sweden) was performed for erythromycin, ciprofloxacin and tetracycline on Mueller-Hinton agar supplemented with 5% sheep blood according to the manufacture's instructions. Inocula were prepared by incubating the strains for 24 h at 42°C under microaerobic conditions in trypticase soy broth. After application of the E-test strips, plates were incubated in microaerobic conditions at 42°C for 48 h. The minimal inhibition concentration (MIC) was read directly from the test strip at the point where the elliptical zone of inhibition intersected the MIC scale on the strip. The following NCCLS zone diameter (mm) and MIC breakpoints for resistance were applied: erythromycin ≤ 13 mm and MIC ≥ 8 mg/l, ciprofloxacin ≤ 15 mm and MIC ≥ 4 mg/l, tetracycline ≤ 14 mm and MIC ≥ 16 mg/l, streptomycin ≤ 11 mm, ampicillin ≤ 13 mm, gentamicin ≤ 12 mm, amoxicillin ≤ 13 mm and sulphonamide ≤ 12 mm.

Statistical analysis consisted of initial bivariate screening for possible risk factors associated with resistance to the tested antibiotics. Variables screened for were:

• production system: free range production or production with bedding and winter garden, labelled as 'animal friendly' vs. conventional production

• foreign production vs. Swiss production

• whole chicken vs. parts of chicken

• products sold pre-packed vs. products sold in open displays

• chilled products vs. frozen products

A χ2 test was applied to determine significance in bivariate screening. Factors significantly associated with antibiotic resistance (p < 0.05) were included into a multiple logistic regression model. Variable selection was performed by stepwise backward selection. Only significant variables (p < 0.05) were retained in the model. For each tested antibiotic, a separate regression analysis was performed. In addition, risk factors for multiple resistance, defined as resistance to at least two of the tested antibiotics, were determined in a separate regression model. All statistical analyses were performed with the statistical software package NCSS 2000 (Number Cruncher Statistical Systems, Kaysville, Utah, USA).

Results

Samples

Overall, 415 samples of raw poultry meat products were purchased from 122 retail shops and cultured for Campylobacter spp. Information about housing type of poultry, processing, packing, refrigeration, and the country of origin of the sampled poultry products is given in table 1.

Table 1

Poultry meat products, purchased from retail. Description of sampled poultry meat products (n = 415), purchased from 122 retail shops.

Raw poultry meat samples (n = 415)
Product category	Sample size	%
Conventional production	240	58%
'Animal-friendly' production	175	42%
Samples from whole chickens	178	43%
Samples from parts of chicken	237	57%
Poultry sold pre-packed	308	74%
Poultry sold in open displays	107	26%
Frozen poultry	76	18%
Chilled poultry	339	82%
Swiss production	296	71%
Imported products	119	29%
Country of origin: France		46
Hungary		20
Italy		19
Germany		17
Denmark		13
Other countries		4

Campylobacter prevalence

Ninety-one out of 415 (21.9%) raw poultry meat samples were found to be positive for Campylobacter spp. As the meat samples were purchased only between March and July, no seasonal trend for prevalence of positive samples could be observed. There was no statistically significant difference between housing types. The prevalence of Campylobacter positive samples was 20.4% for conventionally produced poultry, and 24% for 'animal-friendly' produced poultry. Furthermore, no statistically significant difference was found for the origin of the meat (21% for imported products and 22.3% for Swiss products), the product categories (21.3% for samples from whole chicken and 22.4% for samples from parts of chicken), and the package (21.4% for pre-packed products and 23.4% for products sold in open displays). Frozen products had a statistically significant lower prevalence of Campylobacter positive samples than chilled products (7.9% and 25.1%, respectively; OR = 4.3; 95%CI 1.8–10.3).

Susceptibility testing

In total, 87 Campylobacter strains were isolated for susceptibility testing. Fifty-one strains (58.6%) were sensitive to all tested antibiotics, 36 strains (41.4%) were resistant to at least one of the antibiotics. Nineteen strains (21.8%) were resistant to a single and nine strains to two antibiotics. Triple resistance was shown four times. Two strains were observed with fourfold and fivefold resistance, respectively. The resistance pattern of a fivefold resistant strain showed a striking combination of ciprofloxacin, tetracycline and erythromycin, the most important antibiotics for treatment of human campylobacteriosis. Overall, in multiple resistant isolates, patterns of resistance were heterogeneous.

In figure 1 the results of the sampled retail shops are shown with a possible classification.

Figure 1

Distribution and classification of retail shops. Distribution and classification of the 122 retail shops included in this study. Green dots: shops without Campylobacter-positive samples. Yellow dots: shops with at least one Campylobacter-positive sample; no antibiotic resistance. Orange dots: shops with at least one sample with Campylobacter resistant to one of the tested antibiotics. Red dots: shops with at least one sample with Campylobacter resistant to more than one antibiotic.

Using the disc diffusion method, nine (10.3%) out of 87 strains were resistant to ampicillin, twenty-five (28.7%) to ciprofloxacin, one to erythromycin (1.1%), eleven (12.6%) to tetracycline, seven (8.0%) to streptomycin and none to gentamicin. Five strains (5.9%) out of 85 tested were resistant to amoxicillin and nine (11.8%) out of 76 tested resistant to sulphonamide.

The results of susceptibility testing using the E-test method are shown in figure 2. The comparison of the results for ciprofloxacin and erythromycin showed no differences between the disc diffusion and the E-test method, whereas for tetracycline, there was one strain marginally classified as resistant using the disc diffusion method but susceptible using the E-test method.

Figure 2

Results of E-test method. Results of susceptibility testing to ciprofloxacin, erythromycin and tetracycline using the E-test method. Susceptible strains are marked in blue. Strains with a minimal inhibition concentration (MIC) higher than the breakpoint value for resistance are marked in red.

Risk factor analysis

Figure 3 provides an overview of antibiotic resistance in isolates from 'animal-friendly' and conventional production, and from Swiss and foreign products, respectively. In bivariate analysis "conventional production system vs. 'animal-friendly' production system" (OR = 5.2; 95%CI 1.4–19.8) was one of two significant risk factors for resistance to at least two antibiotics. The second factor, "foreign production vs. Swiss production", was the only risk factor remaining significant for multiple resistance in the logistic regression model (OR = 5.7; 95%CI 1.8–17.7). Risk factors for resistance to ampicillin, amoxicillin, ciprofloxacin, and tetracycline are shown in table 2. No significant risk factors could be identified for resistance in Campylobacter strains to erythromycin, streptomycin and sulphonamide.

Figure 3

Resistance according to production system and to origin. Comparison of antibiotic resistance in Campylobacter spp. isolated from conventional (n = 50) and, animal-friendly' (n = 37) products and from foreign (n = 24) and Swiss (n = 63) products, respectively.

Table 2

Risk factors for antibiotic resistance. Significant risk factors (p < 0.05) for Campylobacter resistance to ciprofloxacin, tetracycline, ampicillin, and amoxicillin, ascertained by logistic regression models.

Antibiotic	Variable	Odds Ratio	95 %-confidence interval	P
Ciprofloxacin	foreign vs. Swiss production	5.0	1.8 – 13.9	0.001
Tetracycline	foreign vs. Swiss production	10.0	2.4 – 42.1	0.002
Ampicillin	foreign vs. Swiss production	6.7	1.5 – 29.4	0.01
Amoxicillin	sale in open displays vs. pre-packed	11.2	1.2 – 106.3	0.03

Discussion

The high prevalence of Campylobacter spp. in raw poultry meat samples found in this study agrees with data from other studies [17-19]. Compared to the situation in other countries, the data of this study show a favourable resistance situation for Campylobacter strains isolated from raw poultry meat produced in Switzerland. In the USA, 90% of Campylobacter strains isolated from poultry meat had resistance to at least one and 45% to at least two antibiotics [20].

In our study, 28.7% of the isolated strains were resistant to ciprofloxacin. This prevalence is comparable to the results reported in the cited American study [20]. A much higher prevalence of ciprofloxacin resistant strains was found in Austria (47%) [21], while a lower prevalence was reported in Denmark (6%) [22]. However, it has to be considered, that in our study the resistance to ciprofloxacin was significantly higher in Campylobacter spp. isolated from foreign products than from Swiss products. Nevertheless, the prevalence of ciprofloxacin resistance in Campylobacter strains from Swiss poultry meat products was 19% and thus higher than for any other antibiotic tested. Data on resistance patterns of Campylobacter isolated from humans in Switzerland only have limited validity (small number of non-representative samples). Nevertheless, the highest rates for resistance, in the last four years, were observed for nalidixid acid (over 80% of isolates) [23]. As there is strong cross resistance to fluoroquinolones which are most frequently used for empiric antimicrobial treatment of acute diarrhoea, this fact gives cause for concern. In Switzerland Baytril®(enrofloxacin) is licensed for therapeutic use in poultry and therefore, ongoing monitoring is required. Twelve point six percent of the isolated strains in our study were resistant against tetracycline. A much higher prevalence of tetracycline resistant strains was found in USA (66%), and in Austria (47%) [20,21]. Only 7% of the strains in the Danish study were tetracycline resistant [22].

Comparable to our data, a low resistance prevalence against erythromycin was also reported in Austria and Denmark [21,22], whereas in the USA 20% erythromycin resistant strains were found [20].

In a recent Swiss study resistance patterns of Campylobacter strains isolated from finishing pigs were investigated [24]. Whereas prevalence of resistance against ciprofloxacin, tetracycline, and gentamicin in these strains was similar to the results of this study, prevalence of resistance against erythromycin and streptomycin was 19.8% and 79.1%, respectively, and therefore much higher than in strains isolated from poultry meat. This finding is consistent with Danish data [25] finding a higher prevalence of resistance to streptomycin in Campylobacter coli, predominantly isolated in pigs, than in Campylobacter jejuni, the predominantly strain in poultry meat. Generally, the comparison of different studies turned out to be difficult because of different sample collection and varieties in culture methods and susceptibility test methods.

A noteworthy finding was the apparent clustering of retail shops with at least one sample with Campylobacter resistant to multiple antibiotics in the south of Switzerland (figure 1). This could be explained by the high proportion of imported poultry meat products in this region. However, due to sample size limitations, analysis by country of origin did not produce meaningful results. Moreover, the results of this study indicate an association between Campylobacter spp. being resistant to amoxicillin and poultry products sold in open displays. One could speculate that this astonishing result may be based on a different origin of the products. Samples from products sold in open displays were predominantly from small local butcheries. Pre-packed products, on the other hand, were primarily sampled in supermarkets. However, no data are available on the patterns of antibiotics use in poultry farms delivering to small butcheries or to supermarkets.

It is known that poultry from free range housing systems have a higher risk of being infected with Campylobacter spp. than poultry from conventional housing systems [26]. However, there was no significant association between these systems and the occurrence of antibiotic resistance in the isolated strains. While in bivariate analysis, both "conventional production vs. 'animal-friendly' production" and "foreign production vs. Swiss production" were significant risk factors for multiple resistance, only "foreign production vs. Swiss production" remained significant in the logistic regression model. This means that the univariate results were caused by confounding as the majority of 'animal-friendly' samples was produced in Switzerland.

Conclusions

In this study, the overall prevalence of ciprofloxacin resistance in Campylobacter strains from raw poultry meat (29%) was higher than the prevalence of ciprofloxacin resistance in Campylobacter strains from poultry meat produced in Switzerland (19%) and foreign versus Swiss production was a significant risk factor for multiple resistant strains in the logistic regression model. Therefore, a national monitoring program for antibiotic resistance needs to include both domestically produced and imported meat.

Competing interests

None declared.

Authors' contributions

UL performed the statistical analysis and drafted the manuscript. GR participated in the study design, coordinated the collaboration with the cantonal laboratories and supervised data analysis. RS was responsible for isolation and susceptibility testing of Campylobacter species. JD and BB participated in the design of the study. JD initiated the collaboration with the cantonal laboratories, and consulted on all practical aspects of sample collection. KS conceived of the project, and provided valuable scientific input during the design, implementation and data analysis phase. All authors read, commented on and approved of the final manuscript.

Pre-publication history

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