| Literature DB >> 14629113 |
Danyel G J Jennen1, Richard P M A Crooijmans, Bram Kamps, Rukiye Açar, Jan J van der Poel, Martien A M Groenen.
Abstract
The physical and comparative map of GGA15 was improved by the construction of 9 BAC contigs around loci previously mapped on GGA15 by linkage analysis. In total, 240 BAC clones were isolated, covering 30-35% of GGA15, and 120 STS were developed (104 STS derived from BAC end sequences and 18 STS derived within genes). Seventeen chicken orthologues of human genes located on human Chr 22q11-q12 were directly mapped within BAC contigs of GGA15. Furthermore, the partial sequences of the chicken BAC clones were compared with sequences present in the EMBL/GenBank databases and revealed matches to 26 genes, ESTs, and genomic clones located on HSA22q11-q12 and HSA12q24. These results provide a better alignment of GGA15 with the corresponding regions in human and mouse, and improve our knowledge of the evolution and dynamics of the vertebrate genome.Entities:
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Year: 2003 PMID: 14629113 DOI: 10.1007/s00335-003-3007-z
Source DB: PubMed Journal: Mamm Genome ISSN: 0938-8990 Impact factor: 2.957