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Literature DB >> 14627809

Cleavage of DNA without loss of genetic information by incorporation of a disaccharide nucleoside.

Koen Nauwelaerts¹, Karen Vastmans, Matheus Froeyen, Veerle Kempeneers, Jef Rozenski, Helmut Rosemeyer, Arthur Van Aerschot, Roger Busson, Jeffrey C Lacey, Ekaterina Efimtseva, Sergey Mikhailov, Eveline Lescrinier, Piet Herdewijn.

Abstract

A ribose residue inserted between the 3'-OH of one nucleotide and the 5'-phosphate group of the next nucleotide, functions as a site-specific cleavage site within DNA. This extra ribose does not interrupt helix formation and it protects duplex DNA against cleavage by restriction enzymes. Cleavage can be obtained with periodate and all ribose fragments can be removed with sodium hydroxide. As a result of this, an intact natural oligodeoxynucleotide is obtained after ligation reaction, which means that site-specific cleavage and recovering of intact DNA occurs without loss of genetic information.

Entities: Chemical

Mesh：

Substances：

Year: 2003 PMID： 14627809 PMCID： PMC290278 DOI： 10.1093/nar/gkg911

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

15 in total

Review 1. RNA structure and NMR spectroscopy.

Authors: G Varani; I Tinoco
Journal: Q Rev Biophys Date: 1991-11 Impact factor: 5.318

2. Crystal structures of open and closed forms of binary and ternary complexes of the large fragment of Thermus aquaticus DNA polymerase I: structural basis for nucleotide incorporation.

Authors: Y Li; S Korolev; G Waksman
Journal: EMBO J Date: 1998-12-15 Impact factor: 11.598

3. Torsion-angle molecular dynamics as a new efficient tool for NMR structure calculation.

Authors: E G Stein; L M Rice; A T Brünger
Journal: J Magn Reson Date: 1997-01 Impact factor: 2.229

4. DNA duplexes with reactive dialdehyde groups as novel reagents for cross-linking to restriction- modification enzymes.

Authors: M G Brevnov; O M Gritsenko; S N Mikhailov; E V Efimtseva; B S Ermolinsky; A Van Aerschot; P Herdewijn; A V Repyk; E S Gromova
Journal: Nucleic Acids Res Date: 1997-08-15 Impact factor: 16.971

5. Palindromic octa- and dodecanucleotides containing 2'-deoxytubercidin: synthesis, hairpin formation, and recognition by the endodeoxyribonuclease EcoRI.

Authors: F Seela; A Kehne
Journal: Biochemistry Date: 1987-04-21 Impact factor: 3.162

1. Incorporation of a disaccharide nucleoside into the backbone of double-stranded DNA: crystallization and preliminary X-ray diffraction.

Authors: Kristof Van Hecke; Koen Uytterhoeven; Sergey N Mikhailov; Piet Herdewijn; Luc Van Meervelt
Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun Date: 2005-09-30

1 in total

Cleavage of DNA without loss of genetic information by incorporation of a disaccharide nucleoside.

Review 1. RNA structure and NMR spectroscopy.

2. Crystal structures of open and closed forms of binary and ternary complexes of the large fragment of Thermus aquaticus DNA polymerase I: structural basis for nucleotide incorporation.

3. Torsion-angle molecular dynamics as a new efficient tool for NMR structure calculation.

4. DNA duplexes with reactive dialdehyde groups as novel reagents for cross-linking to restriction- modification enzymes.

5. Palindromic octa- and dodecanucleotides containing 2'-deoxytubercidin: synthesis, hairpin formation, and recognition by the endodeoxyribonuclease EcoRI.

6. An attempt to unify the structure of polymerases.

7. Improved spectral resolution in cosy 1H NMR spectra of proteins via double quantum filtering.

8. Oligodeoxyribonucleotides containing 1,3-propanediol as nucleoside substitute.

9. Assignment of the 31P and 1H resonances in oligonucleotides by two-dimensional NMR spectroscopy.

10. Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions.

1. Incorporation of a disaccharide nucleoside into the backbone of double-stranded DNA: crystallization and preliminary X-ray diffraction.