Literature DB >> 14624538

Spectroscopy and reactivity of a photogenerated tryptophan radical in a structurally defined protein environment.

Jeremiah E Miller1, Cristian Grădinaru, Brian R Crane, Angel J Di Bilio, William A Wehbi, Sun Un, Jay R Winkler, Harry B Gray.   

Abstract

Near-UV irradiation of structurally characterized [Re(I)(CO)3(1,10-phenanthroline)(Q107H)](W48F/Y72F/H83Q/Y108W)AzM(II) [Az = Pseudomonas aeruginosa azurin, M = Cu, Zn]/[Co(NH3)5Cl]Cl2 produces a tryptophan radical (W108*) with unprecedented kinetic stability. After rapid formation (k = 2.8 x 106 s-1), the radical persists for more than 5 h at room temperature in the folded ReAzM(II) structure. The absorption spectrum of ReAz(W108*)M(II) exhibits maxima at 512 and 536 nm. Oxidation of K4[Mo(CN)8] by ReAz(W108*)Zn(II) places the W108*/W108 reduction potential in the protein above 0.8 V vs NHE.

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Year:  2003        PMID： 14624538     DOI： 10.1021/ja037203i

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  13 in total

1.  Hydrogen bonding of tryptophan radicals revealed by EPR at 700 GHz.

Authors:  Stefan Stoll; Hannah S Shafaat; J Krzystek; Andrew Ozarowski; Michael J Tauber; Judy E Kim; R David Britt
Journal:  J Am Chem Soc       Date:  2011-10-25       Impact factor: 15.419

2.  Photogeneration and Quenching of Tryptophan Radical in Azurin.

Authors:  Bethany C Larson; Jennifer R Pomponio; Hannah S Shafaat; Rachel H Kim; Brian S Leigh; Michael J Tauber; Judy E Kim
Journal:  J Phys Chem B       Date:  2015-02-17       Impact factor: 2.991

3.  In Vitro Anticancer Activity and in Vivo Biodistribution of Rhenium(I) Tricarbonyl Aqua Complexes.

Authors:  Kevin M Knopf; Brendan L Murphy; Samantha N MacMillan; Jeremy M Baskin; Martin P Barr; Eszter Boros; Justin J Wilson
Journal:  J Am Chem Soc       Date:  2017-09-26       Impact factor: 15.419

4.  The Color of Cation-π Interactions: Subtleties of Amine-Tryptophan Interaction Energetics Allow for Radical-like Visible Absorbance and Fluorescence.

Authors:  Laura J Juszczak; Azaria S Eisenberg
Journal:  J Am Chem Soc       Date:  2017-06-07       Impact factor: 15.419

5.  Mass spectrometric characterization of oligomers in Pseudomonas aeruginosa azurin solutions.

Authors:  Lucie Sokolová; Heather Williamson; Jan Sýkora; Martin Hof; Harry B Gray; Bernd Brutschy; Antonín Vlcek
Journal:  J Phys Chem B       Date:  2011-03-31       Impact factor: 2.991

6.  Characterization of NocL involved in thiopeptide nocathiacin I biosynthesis: a [4Fe-4S] cluster and the catalysis of a radical S-adenosylmethionine enzyme.

Authors:  Qi Zhang; Dandan Chen; Jun Lin; Rijing Liao; Wei Tong; Zhinan Xu; Wen Liu
Journal:  J Biol Chem       Date:  2011-03-29       Impact factor: 5.157

Review 7.  Proton-coupled electron flow in protein redox machines.

Authors:  Jillian L Dempsey; Jay R Winkler; Harry B Gray
Journal:  Chem Rev       Date:  2010-11-17       Impact factor: 60.622

8.  Phototriggering electron flow through Re(I)-modified Pseudomonas aeruginosa azurins.

Authors:  Ana María Blanco-Rodríguez; Angel J Di Bilio; Crystal Shih; Anna Katrine Museth; Ian P Clark; Michael Towrie; Andrea Cannizzo; Jawahar Sudhamsu; Brian R Crane; Jan Sýkora; Jay R Winkler; Harry B Gray; Stanislav Záliš; Antonín Vlček
Journal:  Chemistry       Date:  2011-04-05       Impact factor: 5.236

9.  L-tryptophan radical cation electron spin resonance studies: connecting solution-derived hyperfine coupling constants with protein spectral interpretations.

Authors:  Henry D Connor; Bradley E Sturgeon; Carolyn Mottley; Herbert J Sipe; Ronald P Mason
Journal:  J Am Chem Soc       Date:  2008-04-24       Impact factor: 15.419

10.  Spectroscopic evidence for an engineered, catalytically active Trp radical that creates the unique reactivity of lignin peroxidase.

Authors:  Andrew T Smith; Wendy A Doyle; Pierre Dorlet; Anabella Ivancich
Journal:  Proc Natl Acad Sci U S A       Date:  2009-09-14       Impact factor: 11.205
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