Literature DB >> 14607879

Effect of antigen coating conditions on enzyme-linked immunosorbent assay for detection of immunoglobulin G antibody to Neisseria meningitidis serogroup Y and W135 capsular polysaccharide antigens in serum.

Peter C Giardina1, Renee E Evans, Daniel J Sikkema, Dace Madore, Stephen W Hildreth.   

Abstract

Human sera collected from 28 consenting adult volunteers were used to define assay conditions for meningococcal vaccine clinical trial serology. Immunoassay parameters were optimized with these test sera and the standard reference serum, CDC1992. Coating conditions for serogroup Y and W135 polysaccharide antigens were found to influence the predicted serum immunoglobulin G (IgG) antibody concentrations. Sera that displayed IgG antibody binding profiles most unlike that of CDC1992 were influenced the most by coating conditions. Our results suggest that presentation of specific epitopes is influenced by antigen-coating concentrations for serogroup Y and W135 polysaccharides.

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Year:  2003        PMID： 14607879      PMCID： PMC262445          DOI： 10.1128/cdli.10.6.1136-1140.2003

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN： 1071-412X


  24 in total

1.  Enzyme-linked immunosorbent assay. II. Quantitative assay of protein antigen, immunoglobulin G, by means of enzyme-labelled antigen and antibody-coated tubes.

Authors:  E Engvall; K Jonsson; P Perlmann
Journal:  Biochim Biophys Acta       Date:  1971-12-28

2.  Assignment of additional anticapsular antibody concentrations to the Neisseria meningitidis group A, C, Y, and W-135 meningococcal standard reference serum CDC1992.

Authors:  Cheryl M Elie; Patricia K Holder; Sandra Romero-Steiner; George M Carlone
Journal:  Clin Diagn Lab Immunol       Date:  2002-05

3.  A fractionating column for analysis of nucleic acids.

Authors:  J D MANDELL; A D HERSHEY
Journal:  Anal Biochem       Date:  1960-06       Impact factor: 3.365

4.  O-Acetylation status of the capsular polysaccharides of serogroup Y and W135 meningococci isolated in the UK.

Authors:  Emma Longworth; Phil Fernsten; Terri L Mininni; Ulrich Vogel; Heike Claus; Steve Gray; Edward Kaczmarski; Ray Borrow
Journal:  FEMS Immunol Med Microbiol       Date:  2002-01-14

5.  Relationship between serum bactericidal activity and serogroup-specific immunoglobulin G concentration for adults, toddlers, and infants immunized with Neisseria meningitidis serogroup C vaccines.

Authors:  D J Sikkema; K E Friedman; B Corsaro; A Kimura; S W Hildreth; D V Madore; S A Quataert
Journal:  Clin Diagn Lab Immunol       Date:  2000-09

6.  Studies on the meningococcal polysaccharides. II. Composition and chemical properties of the group B and group C polysaccharide.

Authors:  T Y Liu; E C Gotschlich; F T Dunne; E K Jonssen
Journal:  J Biol Chem       Date:  1971-08-10       Impact factor: 5.157

7.  Studies on the meningococcal polysaccharides. I. Composition and chemical properties of the group A polysaccharide.

Authors:  T Y Liu; E C Gotschlich; E K Jonssen; J R Wysocki
Journal:  J Biol Chem       Date:  1971-05-10       Impact factor: 5.157

8.  Human immunity to the meningococcus. IV. Immunogenicity of group A and group C meningococcal polysaccharides in human volunteers.

Authors:  E C Gotschlich; I Goldschneider; M S Artenstein
Journal:  J Exp Med       Date:  1969-06-01       Impact factor: 14.307

9.  Human immunity to the meningococcus. 3. Preparation and immunochemical properties of the group A, group B, and group C meningococcal polysaccharides.

Authors:  E C Gotschlich; T Y Liu; M S Artenstein
Journal:  J Exp Med       Date:  1969-06-01       Impact factor: 14.307

10.  Human immunity to the meningococcus. I. The role of humoral antibodies.

Authors:  I Goldschneider; E C Gotschlich; M S Artenstein
Journal:  J Exp Med       Date:  1969-06-01       Impact factor: 14.307
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  2 in total

1.  Analysis of human serum immunoglobulin G against O-acetyl-positive and O-acetyl-negative serogroup W135 meningococcal capsular polysaccharide.

Authors:  Peter C Giardina; Emma Longworth; Renee E Evans-Johnson; Michaelene L Bessette; Hong Zhang; Ray Borrow; Dace Madore; Philip Fernsten
Journal:  Clin Diagn Lab Immunol       Date:  2005-05

2.  Avidity of the immunoglobulin G response to a Neisseria meningitidis group C polysaccharide conjugate vaccine as measured by inhibition and chaotropic enzyme-linked immunosorbent assays.

Authors:  Shannon L Harris; How Tsao; Lindsey Ashton; David Goldblatt; Philip Fernsten
Journal:  Clin Vaccine Immunol       Date:  2007-02-07
  2 in total

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