| Literature DB >> 11452886 |
E Engvall1, K Jonsson, P Perlmann.
Abstract
Alkaline phosphatase from calf intestinal mucosa has been conjugated to a protein antigen, rabbit IgG. Such conjugates, prepared by glutardialdehyde, have been used in a competitive solid phase immunoassay. In this test native antigen inhibits the binding of the conjugate to homologous antibodies adsorbed to plastic tubes. Using this assay 1-100 ng/ml of the antigen could be determined.Entities:
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Year: 1971 PMID： 11452886 DOI： 10.1016/0005-2795(71)90132-2
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002