Literature DB >> 14605172

Evaluation of acridine orange staining as a replacement of subcultures for BacT/ALERT-positive, gram stain-negative blood cultures.

Hildegard Adler1, Nathalie Baumlin, Reno Frei.   

Abstract

Among 18,424 blood culture sets processed during a study period of 18 months, 85 bottles that were positive by the BacT/ALERT system were Gram stain negative. Both acridine orange staining and subcultures detected microorganisms in a total of 12 bottles. Acridine orange staining can replace subcultures of false-positive blood cultures.

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Year:  2003        PMID： 14605172      PMCID： PMC262529          DOI： 10.1128/JCM.41.11.5238-5239.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  8 in total

1.  Clinical comparison of nonvented aerobic BacT/Alert blood culture bottle and standard aerobic bottle for detection of microorganisms in blood.

Authors:  J W Snyder; K S Benzing; G K Munier; G D Bostic; P S Bozigar; R Hanna; M J Zervos
Journal:  J Clin Microbiol       Date:  2000-10       Impact factor: 5.948

2.  Evaluation of a plastic nonvented aerobic blood culture bottle for use with the BacT/ALERT microbial detection system.

Authors:  J W Snyder; G K Munier; G D Bostic; P S Bozigar; R Hanna
Journal:  J Clin Microbiol       Date:  2002-12       Impact factor: 5.948

3.  Detection and treatment of bloodstream infection: laboratory reporting and antimicrobial management.

Authors:  Erik L Munson; Daniel J Diekema; Susan E Beekmann; Kimberle C Chapin; Gary V Doern
Journal:  J Clin Microbiol       Date:  2003-01       Impact factor: 5.948

4.  The clinical significance of positive blood cultures in the 1990s: a prospective comprehensive evaluation of the microbiology, epidemiology, and outcome of bacteremia and fungemia in adults.

Authors:  M P Weinstein; M L Towns; S M Quartey; S Mirrett; L G Reimer; G Parmigiani; L B Reller
Journal:  Clin Infect Dis       Date:  1997-04       Impact factor: 9.079

5.  Controlled evaluation of BacT/Alert standard aerobic and FAN aerobic blood culture bottles for detection of bacteremia and fungemia.

Authors:  M P Weinstein; S Mirrett; L G Reimer; M L Wilson; S Smith-Elekes; C R Chuard; K L Joho; L B Reller
Journal:  J Clin Microbiol       Date:  1995-04       Impact factor: 5.948

6.  Subcultures of BACTEC-positive but gram or acridine orange stain-negative NR 6A and 7A blood culture bottles are unnecessary.

Authors:  L J Harrell; S Mirrett; L B Reller
Journal:  Diagn Microbiol Infect Dis       Date:  1994-11       Impact factor: 2.803

7.  Controlled comparison of original vented aerobic fan medium with new nonvented BacT/ALERT FA medium for culturing blood.

Authors:  S Mirrett; R J Everts; L B Reller
Journal:  J Clin Microbiol       Date:  2001-06       Impact factor: 5.948

8.  Acridine orange staining as a replacement for subculturing of false-positive blood cultures with the BACTEC NR 660.

Authors:  J S Hunter
Journal:  J Clin Microbiol       Date:  1993-02       Impact factor: 5.948
  8 in total

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