Literature DB >> 14576334

Efficient labeling of fission yeast Schizosaccharomyces pombe with thymidine and BUdR.

Jeffrey A Hodson1, Julie M Bailis, Susan L Forsburg.   

Abstract

In this paper we report the construction of a Schizosaccharomyces pombe strain that facilitates analysis of replicating DNA. The strain co-expresses the Herpes simplex virus thymidine kinase gene (hsv-tk) and a human equilibrative nucleoside transporter (hENT1). The double integrant efficiently incorporates 3H-thymidine into nuclear DNA as monitored by scintillation counting. These strains also incorporate the thymidine analog Bromodeoxy uridine (BUdR) into newly replicated DNA, which can be detected by immunofluorescence and flow cytometry. This strain provides a valuable tool for direct study of DNA replication in S.pombe.

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Year:  2003        PMID： 14576334      PMCID： PMC275491          DOI： 10.1093/nar/gng134

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  35 in total

Review 1.  Making sense of eukaryotic DNA replication origins.

Authors:  D M Gilbert
Journal:  Science       Date:  2001-10-05       Impact factor: 47.728

2.  Functions of fission yeast orp2 in DNA replication and checkpoint control.

Authors:  J Kiely; S B Haase; P Russell; J Leatherwood
Journal:  Genetics       Date:  2000-02       Impact factor: 4.562

3.  Thymidine kinase: evidence for its absence from Neurospora crassa and some other micro-organisms, and the relevance of this to the specific labelling of deoxyribonucleic acid.

Authors:  A R Grivell; J F Jackson
Journal:  J Gen Microbiol       Date:  1968-12

Review 4.  The structure and function of yeast ARS elements.

Authors:  C S Newlon; J F Theis
Journal:  Curr Opin Genet Dev       Date:  1993-10       Impact factor: 5.578

5.  Functional production and reconstitution of the human equilibrative nucleoside transporter (hENT1) in Saccharomyces cerevisiae. Interaction of inhibitors of nucleoside transport with recombinant hENT1 and a glycosylation-defective derivative (hENT1/N48Q).

Authors:  M F Vickers; R S Mani; M Sundaram; D L Hogue; J D Young; S A Baldwin; C E Cass
Journal:  Biochem J       Date:  1999-04-01       Impact factor: 3.857

6.  Thymidine utilization by tut mutants and facile cloning of mutant alleles by plasmid conversion in S. cerevisiae.

Authors:  R A Sclafani; W L Fangman
Journal:  Genetics       Date:  1986-11       Impact factor: 4.562

7.  Functional production of mammalian concentrative nucleoside transporters in Saccharomyces cerevisiae.

Authors:  M F Vickers; J D Young; S A Baldwin; M J Ellison; C E Cass
Journal:  Mol Membr Biol       Date:  2001 Jan-Mar       Impact factor: 2.857

8.  Bromodeoxyuridine 5'-monophosphate incorporation into yeast nuclear and mitochondrial deoxyribonucleic acid.

Authors:  J Leff; K B Lam
Journal:  J Bacteriol       Date:  1976-07       Impact factor: 3.490

9.  Schizosaccharomyces pombe Mcm3p, an essential nuclear protein, associates tightly with Nda4p (Mcm5p).

Authors:  D A Sherman; S L Forsburg
Journal:  Nucleic Acids Res       Date:  1998-09-01       Impact factor: 16.971

10.  Fission yeast cdc21, a member of the MCM protein family, is required for onset of S phase and is located in the nucleus throughout the cell cycle.

Authors:  D Maiorano; G B Van Assendelft; S E Kearsey
Journal:  EMBO J       Date:  1996-02-15       Impact factor: 11.598
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  31 in total

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Authors:  Zhongsheng You; Charly Chahwan; Julie Bailis; Tony Hunter; Paul Russell
Journal:  Mol Cell Biol       Date:  2005-07       Impact factor: 4.272

2.  Differential arrival of leading and lagging strand DNA polymerases at fission yeast telomeres.

Authors:  Bettina A Moser; Lakxmi Subramanian; Ya-Ting Chang; Chiaki Noguchi; Eishi Noguchi; Toru M Nakamura
Journal:  EMBO J       Date:  2009-02-12       Impact factor: 11.598

3.  Incorporation of thymidine analogs for studying replication kinetics in fission yeast.

Authors:  Nicholas Rhind
Journal:  Methods Mol Biol       Date:  2009

4.  Consequences of abnormal CDK activity in S phase.

Authors:  Silje Anda; Christiane Rothe; Erik Boye; Beáta Grallert
Journal:  Cell Cycle       Date:  2016       Impact factor: 4.534

5.  S. pombe replication protein Cdc18 (Cdc6) interacts with Swi6 (HP1) heterochromatin protein: region specific effects and replication timing in the centromere.

Authors:  Pao-Chen Li; Louise Chretien; Jocelyn Côté; Thomas J Kelly; Susan L Forsburg
Journal:  Cell Cycle       Date:  2011-01-15       Impact factor: 4.534

6.  Telomere regulation during the cell cycle in fission yeast.

Authors:  Bettina A Moser; Ya-Ting Chang; Toru M Nakamura
Journal:  Methods Mol Biol       Date:  2014

7.  Meiotic S-phase damage activates recombination without checkpoint arrest.

Authors:  Daniel G Pankratz; Susan L Forsburg
Journal:  Mol Biol Cell       Date:  2005-02-02       Impact factor: 4.138

8.  Global Inhibition with Specific Activation: How p53 and MYC Redistribute the Transcriptome in the DNA Double-Strand Break Response.

Authors:  Joshua R Porter; Brian E Fisher; Laura Baranello; Julia C Liu; Diane M Kambach; Zuqin Nie; Woo Seuk Koh; Ji Luo; Jayne M Stommel; David Levens; Eric Batchelor
Journal:  Mol Cell       Date:  2017-08-31       Impact factor: 17.970

9.  Incorporation of thymidine analogs for studying replication kinetics in fission yeast.

Authors:  Nicholas Rhind
Journal:  Methods Mol Biol       Date:  2015

10.  A mammalian-like DNA damage response of fission yeast to nucleoside analogs.

Authors:  Sarah A Sabatinos; Tara L Mastro; Marc D Green; Susan L Forsburg
Journal:  Genetics       Date:  2012-11-12       Impact factor: 4.562
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