Literature DB >> 14576129

Resazurin microtiter assay plate testing of Mycobacterium tuberculosis susceptibilities to second-line drugs: rapid, simple, and inexpensive method.

Anandi Martin1, Mirtha Camacho, Françoise Portaels, Juan Carlos Palomino.   

Abstract

The emergence of multidrug-resistant tuberculosis calls for new, rapid drug susceptibility tests. We have tested 150 Mycobacterium tuberculosis isolates against the second-line drugs ethionamide, kanamycin, capreomycin, ofloxacin, and para-aminosalicylic acid by the colorimetric resazurin microtiter assay and the proportion method. By visual reading, MICs were obtained after 8 days. A very good correlation between results by the colorimetric resazurin microtiter assay and the proportion method was obtained. The colorimetric resazurin microtiter assay is inexpensive, rapid, and simple to perform, and implementation of the assay is feasible for low-resource countries.

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Year:  2003        PMID： 14576129      PMCID： PMC253784          DOI： 10.1128/AAC.47.11.3616-3619.2003

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  46 in total

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2.  Kanamycin susceptibility testing of Mycobacterium tuberculosis using Mycobacterium Growth Indicator Tube and a colorimetric method.

Authors:  I Bastian; L Rigouts; J C Palomino; F Portaels
Journal:  Antimicrob Agents Chemother       Date:  2001-06       Impact factor: 5.191

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Review 4.  Tuberculosis in patients with HIV infection.

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6.  Implications of the growing HIV-1 epidemic for tuberculosis control in Russia.

Authors:  B Kazionny; C D Wells; H Kluge; N Gusseynova; V Molotilov
Journal:  Lancet       Date:  2001-11-03       Impact factor: 79.321

7.  Determinants of drug-resistant tuberculosis: analysis of 11 countries.

Authors:  M A Espinal; K Laserson; M Camacho; Z Fusheng; S J Kim; R E Tlali; I Smith; P Suarez; M L Antunes; A G George; N Martin-Casabona; P Simelane; K Weyer; N Binkin; M C Raviglione
Journal:  Int J Tuberc Lung Dis       Date:  2001-10       Impact factor: 2.373

8.  Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis.

Authors:  Juan-Carlos Palomino; Anandi Martin; Mirtha Camacho; Humberto Guerra; Jean Swings; Françoise Portaels
Journal:  Antimicrob Agents Chemother       Date:  2002-08       Impact factor: 5.191

9.  MYCOBACTERIA: LABORATORY METHODS FOR TESTING DRUG SENSITIVITY AND RESISTANCE.

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Journal:  Emerg Infect Dis       Date:  2001 Sep-Oct       Impact factor: 6.883
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3.  Rapid detection of ofloxacin resistance in Mycobacterium tuberculosis by two low-cost colorimetric methods: resazurin and nitrate reductase assays.

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5.  Use of colorimetric culture methods for detection of Mycobacterium tuberculosis complex isolates from sputum samples in resource-limited settings.

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6.  Resazurin Microtiter Assay for Clarithromycin Susceptibility Testing of Clinical Isolates of Mycobacterium abscessus Group.

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7.  Coresistance to isoniazid and ethionamide maps to mycothiol biosynthetic genes in Mycobacterium bovis.

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8.  A noncompetitive inhibitor for Mycobacterium tuberculosis's class IIa fructose 1,6-bisphosphate aldolase.

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10.  Comparison of rapid tests for detection of rifampicin-resistant Mycobacterium tuberculosis in Kampala, Uganda.

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