Literature DB >> 14532220

Rapid and specific detection of Mycobacterium tuberculosis by using the Smart Cycler instrument and a specific fluorogenic probe.

Timothy J Cleary1, Gladys Roudel, Ofelia Casillas, Nancimae Miller.   

Abstract

A procedure using the Smart Cycler instrument and a fluorescence quencher (FQ) probe for the specific identification of Mycobacterium tuberculosis complex (MTB) was used to detect organisms in 366 acid-fast bacillus smear-positive respiratory specimens. It was compared to culture and the AMPLICOR M. tuberculosis PCR test. MTB was isolated from 198 of these samples. The FQ PCR assay was sensitive (197 of 198, 99.5%) and specific (165 of 168, 98.2%); no significant difference was observed between the two PCR protocols. After DNA extraction, a final result was available within 1.5 h with the real-time PCR protocol.

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Year:  2003        PMID： 14532220      PMCID： PMC254309          DOI： 10.1128/JCM.41.10.4783-4786.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  20 in total

1.  Removal of PCR inhibitors by silica membranes: evaluating the Amplicor Mycobacterium tuberculosis kit.

Authors:  B Böddinghaus; T A Wichelhaus; V Brade; T Bittner
Journal:  J Clin Microbiol       Date:  2001-10       Impact factor: 5.948

2.  Evaluation of three nucleic acid amplification methods for direct detection of Mycobacterium tuberculosis complex in respiratory specimens.

Authors:  S X Wang; L Tay
Journal:  J Clin Microbiol       Date:  1999-06       Impact factor: 5.948

3.  Controlling tuberculosis in India.

Authors:  G R Khatri; Thomas R Frieden
Journal:  N Engl J Med       Date:  2002-10-31       Impact factor: 91.245

4.  Rapid and specific detection of Mycobacterium tuberculosis from acid-fast bacillus smear-positive respiratory specimens and BacT/ALERT MP culture bottles by using fluorogenic probes and real-time PCR.

Authors:  Nancimae Miller; Tim Cleary; Günter Kraus; Andrea K Young; Gina Spruill; H James Hnatyszyn
Journal:  J Clin Microbiol       Date:  2002-11       Impact factor: 5.948

5.  New real-time PCR able to detect in a single tube multiple rifampin resistance mutations and high-level isoniazid resistance mutations in Mycobacterium tuberculosis.

Authors:  Darío García de Viedma; Maria del Sol Díaz Infantes; Fátima Lasala; Fernando Chaves; Luis Alcalá; Emilio Bouza
Journal:  J Clin Microbiol       Date:  2002-03       Impact factor: 5.948

6.  Use of real-time PCR and fluorimetry for rapid detection of rifampin and isoniazid resistance-associated mutations in Mycobacterium tuberculosis.

Authors:  M J Torres; A Criado; J C Palomares; J Aznar
Journal:  J Clin Microbiol       Date:  2000-09       Impact factor: 5.948

7.  Update: Nucleic acid amplification tests for tuberculosis.

Authors: 
Journal:  MMWR Morb Mortal Wkly Rep       Date:  2000-07-07       Impact factor: 17.586

8.  Rapid and specific detection of the Mycobacterium tuberculosis complex using fluorogenic probes andreal-time PCR.

Authors:  G Kraus; T Cleary; N Miller; R Seivright; A K Young; G Spruill; H J Hnatyszyn
Journal:  Mol Cell Probes       Date:  2001-12       Impact factor: 2.365

9.  Detection of Mycobacterium bovis in bovine clinical specimens using real-time fluorescence and fluorescence resonance energy transfer probe rapid-cycle PCR.

Authors:  M J Taylor; M S Hughes; R A Skuce; S D Neill
Journal:  J Clin Microbiol       Date:  2001-04       Impact factor: 5.948

10.  Rapid-cycle PCR and fluorimetry for detection of mycobacteria.

Authors:  Jacqueline Lachnik; Birgit Ackermann; Antje Bohrssen; Silvia Maass; Catharina Diephaus; Axel Puncken; Marion Stermann; Franz-Christoph Bange
Journal:  J Clin Microbiol       Date:  2002-09       Impact factor: 5.948
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  14 in total

1.  Comparison of the real-time PCR method and the Gen-Probe amplified Mycobacterium tuberculosis direct test for detection of Mycobacterium tuberculosis in pulmonary and nonpulmonary specimens.

Authors:  Nadine Lemaître; Sylvie Armand; Anne Vachée; Odile Capilliez; Christine Dumoulin; René J Courcol
Journal:  J Clin Microbiol       Date:  2004-09       Impact factor: 5.948

Review 2.  Real-time PCR in clinical microbiology: applications for routine laboratory testing.

Authors:  M J Espy; J R Uhl; L M Sloan; S P Buckwalter; M F Jones; E A Vetter; J D C Yao; N L Wengenack; J E Rosenblatt; F R Cockerill; T F Smith
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

Review 3.  Current evidence on diagnostic accuracy of commercially based nucleic acid amplification tests for the diagnosis of pulmonary tuberculosis.

Authors:  S Greco; E Girardi; A Navarra; C Saltini
Journal:  Thorax       Date:  2006-05-31       Impact factor: 9.139

4.  Molecular-beacon-based real-time PCR for detection and quantification of Mycobacterium tuberculosis DNA in clinical samples.

Authors:  Joseph Papaparaskevas; Dimitra P Houhoula; Argirios Siatelis; Athanassios Tsakris
Journal:  J Clin Microbiol       Date:  2008-07-16       Impact factor: 5.948

5.  Diagnostic accuracy of in-house PCR for pulmonary tuberculosis in smear-positive patients: meta-analysis and metaregression.

Authors:  S Greco; M Rulli; E Girardi; C Piersimoni; C Saltini
Journal:  J Clin Microbiol       Date:  2009-01-14       Impact factor: 5.948

6.  Comparison of an internally controlled, large-volume LightCycler assay for detection of Mycobacterium tuberculosis in clinical samples with the COBAS AMPLICOR assay.

Authors:  Siegfried Burggraf; Udo Reischl; Naeem Malik; Markus Bollwein; Ludmila Naumann; Bernhard Olgemöller
Journal:  J Clin Microbiol       Date:  2005-04       Impact factor: 5.948

7.  Comparison of two molecular methods for rapid diagnosis of extrapulmonary tuberculosis.

Authors:  Manuel Causse; Pilar Ruiz; Juan Bautista Gutiérrez-Aroca; Manuel Casal
Journal:  J Clin Microbiol       Date:  2011-06-08       Impact factor: 5.948

8.  Evaluation of the rapid MGIT TBc identification test for culture confirmation of Mycobacterium tuberculosis complex strain detection.

Authors:  Ming-Chih Yu; Huang-Yao Chen; Mei-Hua Wu; Wei-Lun Huang; Yuh-Min Kuo; Fang-Lan Yu; Ruwen Jou
Journal:  J Clin Microbiol       Date:  2010-12-29       Impact factor: 5.948

9.  Multi-probe real-time PCR identification of common Mycobacterium species in blood culture broth.

Authors:  Suporn Foongladda; Suporn Pholwat; Boonchuay Eampokalap; Pattarachai Kiratisin; Ruengpung Sutthent
Journal:  J Mol Diagn       Date:  2008-12-18       Impact factor: 5.568

10.  Evaluation of multiplex real-time PCR for detection of Haemophilus ducreyi, Treponema pallidum, herpes simplex virus type 1 and 2 in the diagnosis of genital ulcer disease in the Rakai District, Uganda.

Authors:  T R Suntoke; A Hardick; A A R Tobian; B Mpoza; O Laeyendecker; D Serwadda; P Opendi; C A Gaydos; R H Gray; M J Wawer; T C Quinn; S J Reynolds
Journal:  Sex Transm Infect       Date:  2008-12-09       Impact factor: 3.519
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