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Literature DB >> 14507724

Measurements of protein-protein interactions by size exclusion chromatography.

Abstract

A method is presented for determining second virial coefficients (B(2)) of protein solutions from retention time measurements in size exclusion chromatography. We determine B(2) by analyzing the concentration dependence of the chromatographic partition coefficient. We show the ability of this method to track the evolution of B(2) from positive to negative values in lysozyme and bovine serum albumin solutions. Our size exclusion chromatography results agree quantitatively with data obtained by light scattering.

Entities: Gene Species

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Year: 2003 PMID： 14507724 PMCID： PMC1303485 DOI： 10.1016/S0006-3495(03)74684-0

Source DB: PubMed Journal: Biophys J ISSN： 0006-3495 Impact factor: 4.033

7 in total

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Journal: Biophys Chem Date: 1999-01-11 Impact factor: 2.352

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Authors: Peter M Tessier; Abraham M Lenhoff; Stanley I Sandler
Journal: Biophys J Date: 2002-03 Impact factor: 4.033

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Journal: Acta Crystallogr D Biol Crystallogr Date: 1994-07-01

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Journal: Biophys J Date: 1998-12 Impact factor: 4.033

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Journal: Biophys Chem Date: 1978-11 Impact factor: 2.352

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Authors: P R Wills; L W Nichol; R J Siezen
Journal: Biophys Chem Date: 1980-02 Impact factor: 2.352

7. Thermodynamic nonideality in macromolecular solutions. Evaluation of parameters for the prediction of covolume effects.

Authors: K E Shearwin; D J Winzor
Journal: Eur J Biochem Date: 1990-07-05

7 in total

11 in total

1. Determination of second virial coefficient of proteins using a dual-detector cell for simultaneous measurement of scattered light intensity and concentration in SEC-HPLC.

Authors: Harminder Bajaj; Vikas K Sharma; Devendra S Kalonia
Journal: Biophys J Date: 2004-10-01 Impact factor: 4.033

2. Diffusion and sedimentation interaction parameters for measuring the second virial coefficient and their utility as predictors of protein aggregation.

Authors: Atul Saluja; R Matthew Fesinmeyer; Sabine Hogan; David N Brems; Yatin R Gokarn
Journal: Biophys J Date: 2010-10-20 Impact factor: 4.033

3. Patterns of protein protein interactions in salt solutions and implications for protein crystallization.

Authors: André C Dumetz; Ann M Snellinger-O'brien; Eric W Kaler; Abraham M Lenhoff
Journal: Protein Sci Date: 2007-09 Impact factor: 6.725

4. Determination of the second virial coefficient of bovine serum albumin under varying pH and ionic strength by composition-gradient multi-angle static light scattering.

Authors: Yingfang Ma; Diana M Acosta; Jon R Whitney; Rudolf Podgornik; Nicole F Steinmetz; Roger H French; V Adrian Parsegian
Journal: J Biol Phys Date: 2014-11-18 Impact factor: 1.365

Review 5. Protein-protein interactions: switch from classical methods to proteomics and bioinformatics-based approaches.

Authors: Armand G Ngounou Wetie; Izabela Sokolowska; Alisa G Woods; Urmi Roy; Katrin Deinhardt; Costel C Darie
Journal: Cell Mol Life Sci Date: 2013-04-12 Impact factor: 9.261

Review 6. The development and characterization of protein-based stationary phases for studying drug-protein and protein-protein interactions.

Authors: Mitesh Sanghvi; Ruin Moaddel; Irving W Wainer
Journal: J Chromatogr A Date: 2011-05-27 Impact factor: 4.759

Review 7. Assessment and significance of protein-protein interactions during development of protein biopharmaceuticals.

Authors: Sandeep Yadav; Jun Liu; Thomas M Scherer; Yatin Gokarn; Barthélemy Demeule; Sonoko Kanai; James D Andya; Steven J Shire
Journal: Biophys Rev Date: 2013-03-14

8. Methodology and applications of disease biomarker identification in human serum.

Authors: Ziad J Sahab; Suzan M Semaan; Qing-Xiang Amy Sang
Journal: Biomark Insights Date: 2007-02-14

9. Virus assembly occurs following a pH- or Ca2+-triggered switch in the thermodynamic attraction between structural protein capsomeres.

Authors: Yap P Chuan; Yuan Y Fan; Linda H L Lua; Anton P J Middelberg
Journal: J R Soc Interface Date: 2009-07-22 Impact factor: 4.118

10. Pneumococcal surface adhesion A protein (PsaA) interacts with human Annexin A2 on airway epithelial cells.

Authors: Yoonsung Hu; Nogi Park; Keun Seok Seo; Joo Youn Park; Radha P Somarathne; Alicia K Olivier; Nicholas C Fitzkee; Justin A Thornton
Journal: Virulence Date: 2021-12 Impact factor: 5.882