Literature DB >> 14500024

A high-throughput capillary assay for bacterial chemotaxis.

Russell Bainer1, Heungwon Park, Philippe Cluzel.   

Abstract

We present a high-throughput capillary assay in order to characterize the chemotactic response of the E. coli bacterium. We measure the number of organisms attracted into an array of 96 capillary tubes containing the attractant L-aspartate. The effect of bacterial concentration on the chemotactic response is reported. Such high-throughput assay can be used to characterize bacterial chemotaxis function of a wide range of biochemical parameters.

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Year:  2003        PMID： 14500024     DOI： 10.1016/s0167-7012(03)00112-x

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  14 in total

1.  Interdependence of behavioural variability and response to small stimuli in bacteria.

Authors:  Heungwon Park; William Pontius; Calin C Guet; John F Marko; Thierry Emonet; Philippe Cluzel
Journal:  Nature       Date:  2010-11-14       Impact factor: 49.962

2.  Continuous-flow capillary assay for measuring bacterial chemotaxis.

Authors:  Aaron M J Law; Michael D Aitken
Journal:  Appl Environ Microbiol       Date:  2005-06       Impact factor: 4.792

3.  Fine-tuning of chemotactic response in E. coli determined by high-throughput capillary assay.

Authors:  Heungwon Park; Calin C Guet; Thierry Emonet; Philippe Cluzel
Journal:  Curr Microbiol       Date:  2010-10-24       Impact factor: 2.188

4.  In-cell protein NMR and protein leakage.

Authors:  Christopher O Barnes; Gary J Pielak
Journal:  Proteins       Date:  2011-02

5.  Chemotaxis by natural populations of coral reef bacteria.

Authors:  Jessica Tout; Thomas C Jeffries; Katherina Petrou; Gene W Tyson; Nicole S Webster; Melissa Garren; Roman Stocker; Peter J Ralph; Justin R Seymour
Journal:  ISME J       Date:  2015-01-23       Impact factor: 10.302

6.  Controlling and quantifying protein concentration in Escherichia coli.

Authors:  Shannon L Speer; Alex J Guseman; Jon B Patteson; Brandie M Ehrmann; Gary J Pielak
Journal:  Protein Sci       Date:  2019-05-22       Impact factor: 6.725

7.  µFBI: a microfluidic bead-based immunoassay for multiplexed detection of proteins from a µL sample volume.

Authors:  Xiaobo Yu; Michael Hartmann; Quan Wang; Oliver Poetz; Nicole Schneiderhan-Marra; Dieter Stoll; Cornelia Kazmaier; Thomas O Joos
Journal:  PLoS One       Date:  2010-10-01       Impact factor: 3.240

8.  Mucin-Degrading Microbes Release Monosaccharides That Chemoattract Clostridioides difficile and Facilitate Colonization of the Human Intestinal Mucus Layer.

Authors:  Melinda A Engevik; Amy C Engevik; Kristen A Engevik; Jennifer M Auchtung; Alexandra L Chang-Graham; Wenly Ruan; Ruth Ann Luna; Joseph M Hyser; Jennifer K Spinler; James Versalovic
Journal:  ACS Infect Dis       Date:  2020-11-11       Impact factor: 5.084

9.  Effects of recombinant protein expression on green fluorescent protein diffusion in Escherichia coli.

Authors:  Kristin M Slade; Rachael Baker; Michael Chua; Nancy L Thompson; Gary J Pielak
Journal:  Biochemistry       Date:  2009-06-16       Impact factor: 3.162

10.  Chemotaxis to pyrimidines and identification of a cytosine chemoreceptor in Pseudomonas putida.

Authors:  Xianxian Liu; Piper L Wood; Juanito V Parales; Rebecca E Parales
Journal:  J Bacteriol       Date:  2009-02-27       Impact factor: 3.490
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