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Literature DB >> 14276120

EGG YOLK FACTOR OF STAPHYLOCOCCUS AUREUS. II. CHARACTERIZATION OF THE LIPASE ACTIVITY.

Abstract

Shah, D. B. (University of Wisconsin, Madison), and J. B. Wilson. Egg yolk factor of Staphylococcus aureus. II. Characterization of the lipase activity. J. Bacteriol. 89:949-953. 1965.-The staphylococcal egg yolk factor was characterized as a lipase. The enzyme had an optimal pH of 7.8, but the optimal pH of stability was 7. Substrate specificity data showed that the relative rate of hydrolysis was lowest with triacetin as substrate, was maximal with tributyrin, and decreased as the chain length of the acyl moieties increased. The enzyme showed an absolute requirement for a fatty acid acceptor like calcium, when the acyl moiety of triglyceride was water-insoluble. Magnesium, strontium, and barium functioned equally well as fatty acid acceptors. The enzyme was able to hydrolyze coconut oil, peanut oil, olive oil, and egg yolk oil.

Entities: Chemical Disease Species

Keywords: CALCIUM; EGG YOLK; ENZYME INHIBITORS; EXPERIMENTAL LAB STUDY; HYDROGEN-ION CONCENTRATION; LIPASE; STAPHYLOCOCCUS

Mesh：

Substances：

Year: 1965 PMID： 14276120 PMCID： PMC277577 DOI： 10.1128/jb.89.4.949-953.1965

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

12 in total

1. Biochemical properties of virulent and avirulent staphylococci.

Authors: J BURNS; D F HOLTMAN
Journal: Ann N Y Acad Sci Date: 1960-11-21 Impact factor: 5.691

2. Pancreatic lipase.

Authors: P DESNUELLE
Journal: Adv Enzymol Relat Subj Biochem Date: 1961

3. Purification of hog pancreatic lipase.

Authors: G MARCHIS-MOUREN; L SARDA; P DESNUELLE
Journal: Arch Biochem Biophys Date: 1959-07 Impact factor: 4.013

4. [Inhibition of pancreatic lipase by diethyl-p-nitrophenyl phosphate in emulation].

Authors: P DESNUELLE; L SARDA; G AILHAUD
Journal: Biochim Biophys Acta Date: 1960-01-29

5. Enzymatic activity of staphylocoagulase. I. Characterization of an esterase associated with purified preparations.

Authors: M C DRUMMOND; M TAGER
Journal: J Bacteriol Date: 1959-09 Impact factor: 3.490

6. Enzymatic activity of staphylocoagulase. II. Dissociation of plasma clotting from tributyrinase activity.

Authors: M C DRUMMOND; M TAGER
Journal: J Bacteriol Date: 1959-09 Impact factor: 3.490

7. [Actions of pancreatic lipase on esters in emulsions].

Authors: L SARDA; P DESNUELLE
Journal: Biochim Biophys Acta Date: 1958-12

8. A study of the staphylococci associated with the bovine udder.

Authors: W B REID; J B WILSON
Journal: Am J Vet Res Date: 1959-09 Impact factor: 1.156

9. Procarboxypeptidase. II. Chromatographic isolation, further characterization, and activation.

Authors: P J KELLER; E COHEN; H NEURATH
Journal: J Biol Chem Date: 1958-02 Impact factor: 5.157

10. EGG YOLK FACTOR OF STAPHYLOCOCCUS AUREUS. I. NATURE OF THE SUBSTRATE AND ENZYME INVOLVED IN THE EGG YOLK OPACITY REACTION.

Authors: D B SHAH; J B WILSON
Journal: J Bacteriol Date: 1963-03 Impact factor: 3.490

14 in total

1. Glycerol Ester Hydrolase Activity of Microbacterium thermosphactum.

Authors: D L Collins-Thompson; T Sørhaug; L D Witter; Z J Ordal
Journal: Appl Microbiol Date: 1971-01

Review 2. Staphylococcal lipases.

Authors: D V Vadehra
Journal: Lipids Date: 1974-03 Impact factor: 1.880

EGG YOLK FACTOR OF STAPHYLOCOCCUS AUREUS. II. CHARACTERIZATION OF THE LIPASE ACTIVITY.

1. Biochemical properties of virulent and avirulent staphylococci.

2. Pancreatic lipase.

3. Purification of hog pancreatic lipase.

4. [Inhibition of pancreatic lipase by diethyl-p-nitrophenyl phosphate in emulation].

5. Enzymatic activity of staphylocoagulase. I. Characterization of an esterase associated with purified preparations.

6. Enzymatic activity of staphylocoagulase. II. Dissociation of plasma clotting from tributyrinase activity.

7. [Actions of pancreatic lipase on esters in emulsions].

8. A study of the staphylococci associated with the bovine udder.

9. Procarboxypeptidase. II. Chromatographic isolation, further characterization, and activation.

10. EGG YOLK FACTOR OF STAPHYLOCOCCUS AUREUS. I. NATURE OF THE SUBSTRATE AND ENZYME INVOLVED IN THE EGG YOLK OPACITY REACTION.

1. Glycerol Ester Hydrolase Activity of Microbacterium thermosphactum.

Review 2. Staphylococcal lipases.

3. Lipase activity of Lactobacillus brevis.

4. Lipolytic esterases in staphylococci.

5. Purification and properties of a glycerol ester hydrolase (lipase) from Propionibacterium shermanii.

6. Isolation and characterization of a staphylococcal lipase.

7. Purification and characterization of extracellular Staphylococcus warneri lipase.

8. Isolation and purification of staphylococcal lipase.

9. Characterization of purified staphylococcal lipase.

10. Thin-layer chromatographic technique for rapid detection of bacterial phospholipases.