Literature DB >> 1422135

Use of resorufin-labelled N-glycopeptide in a high-performance liquid chromatography assay to monitor endoglycosidase activities during cultivation of Flavobacterium meningosepticum.

S Bourgerie1, Y Karamanos, S Berger, R Julien.   

Abstract

Peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase F (PNGase F) and endo-beta-N-acetyl glucosaminidase F (Endo F) activities were monitored during cultivation of Flavobacterium meningosepticum using a new fluorescence-HPLC procedure based on a commercially available substrate. The PNGase F activity reached a maximum level at the end of the log phase and remained constant during the stationary phase, while Endo F continuously increased until late stationary phase. PNGase F obtained at the end of the log phase was less contaminated by other proteins compared with late stationary phase.

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Year:  1992        PMID： 1422135     DOI： 10.1007/bf00731160

Source DB:  PubMed          Journal:  Glycoconj J        ISSN： 0282-0080            Impact factor:   2.916


  14 in total

1.  Peptide: N-glycosidase F: studies on the glycoprotein aminoglycan amidase from Flavobacterium meningosepticum.

Authors:  K J Mussar; G J Murray; B M Martin; T Viswanatha
Journal:  J Biochem Biophys Methods       Date:  1989

2.  Optimized deglycosylation of glycoproteins by peptide-N4-(N-acetyl-beta-glucosaminyl)-asparagine amidase from Flavobacterium meningosepticum.

Authors:  R Nuck; M Zimmermann; D Sauvageot; W Reutter
Journal:  Glycoconj J       Date:  1990       Impact factor: 2.916

3.  Peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase and endo-beta-N-acetylglucosaminidase from Flavobacterium meningosepticum.

Authors:  A L Tarentino; T H Plummer
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

4.  Endoglycosidases from Flavobacterium meningosepticum application to biological problems.

Authors:  S Alexander; J H Elder
Journal:  Methods Enzymol       Date:  1989       Impact factor: 1.600

5.  endo-beta-N-acetylglucosaminidase F: endoglycosidase from Flavobacterium meningosepticum that cleaves both high-mannose and complex glycoproteins.

Authors:  J H Elder; S Alexander
Journal:  Proc Natl Acad Sci U S A       Date:  1982-08       Impact factor: 11.205

6.  Deglycosylation of asparagine-linked glycans by peptide:N-glycosidase F.

Authors:  A L Tarentino; C M Gómez; T H Plummer
Journal:  Biochemistry       Date:  1985-08-13       Impact factor: 3.162

7.  Amino acid analysis by reverse-phase high-performance liquid chromatography: precolumn derivatization with phenylisothiocyanate.

Authors:  R L Heinrikson; S C Meredith
Journal:  Anal Biochem       Date:  1984-01       Impact factor: 3.365

8.  Purification of the oligosaccharide-cleaving enzymes of Flavobacterium meningosepticum.

Authors:  T H Plummer; A L Tarentino
Journal:  Glycobiology       Date:  1991-06       Impact factor: 4.313

9.  Facile cleavage of complex oligosaccharides from glycopeptides by almond emulsin peptide: N-glycosidase.

Authors:  T H Plummer; A L Tarentino
Journal:  J Biol Chem       Date:  1981-10-25       Impact factor: 5.157

10.  Detection and quantification of peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidases.

Authors:  T H Plummer; A W Phelan; A L Tarentino
Journal:  Eur J Biochem       Date:  1987-02-16
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  4 in total

1.  Characterization of the peptide-N4-(N-acetylglucosaminyl) asparagine amidase (PNGase Se) from Silene alba cells.

Authors:  S Lhernould; Y Karamanos; P Lerouge; H Morvan
Journal:  Glycoconj J       Date:  1995-02       Impact factor: 2.916

2.  Purification and characterization of an endo-N-acetyl-beta-D-glucosaminidase from the culture medium of Stigmatella aurantiaca DW4.

Authors:  S Bourgerie; Y Karamanos; T Grard; R Julien
Journal:  J Bacteriol       Date:  1994-10       Impact factor: 3.490

3.  Regulation of De-N-Glycosylation Enzymes in Germinating Radish Seeds.

Authors:  S. Berger; A. Menudier; R. Julien; Y. Karamanos
Journal:  Plant Physiol       Date:  1996-09       Impact factor: 8.340

4.  Use of porcine fibrinogen as a model glycoprotein to study the binding specificity of the three variants of K88 lectin.

Authors:  C L'Hôte; S Berger; S Bourgerie; Y Duval-Iflah; R Julien; Y Karamanos
Journal:  Infect Immun       Date:  1995-05       Impact factor: 3.441
  4 in total

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