Literature DB >> 7928985

Purification and characterization of an endo-N-acetyl-beta-D-glucosaminidase from the culture medium of Stigmatella aurantiaca DW4.

S Bourgerie1, Y Karamanos, T Grard, R Julien.   

Abstract

A novel endo-N-acetyl-beta-D-glucosaminidase (ENGase), acting on the di-N-acetylchitobiosyl part of N-linked glycans, was characterized in the culture medium of Stigmatella aurantiaca DW4. Purified to homogeneity by ammonium sulfate precipitation, gel filtration, and chromatofocusing, this ENGase presents, upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, a molecular mass near 27 kDa. Optimal pH and pI were 4.0 and 6.8, respectively. The enzyme, named ENGase St, exhibits high activity on oligomannoside-type glycoasparagines and glycoproteins and could also hydrolyze hybrid- and complex-type glycoasparagines but does not acts as a murein hydrolase.

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Year:  1994        PMID： 7928985      PMCID： PMC196955          DOI： 10.1128/jb.176.20.6170-6174.1994

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  21 in total

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Authors:  G T Qualls; K Stephens; D White
Journal:  Dev Biol       Date:  1978-09       Impact factor: 3.582

2.  The substrate specificities of endo-beta-N-acetylglucosaminidases CII and H.

Authors:  T Tai; K Yamashita; A Kobata
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3.  Purification and properties of an extracellular protease from Myxococcus virescens.

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Journal:  J Bacteriol       Date:  1978-01       Impact factor: 3.490

4.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  The structures of the galactose-containing sugar chains of ovalbumin.

Authors:  K Yamashita; Y Tachibana; A Kobata
Journal:  J Biol Chem       Date:  1978-06-10       Impact factor: 5.157

7.  Bacteriolytic enzymes produced by Myxococcus xanthus.

Authors:  S Sudo; M Dworkin
Journal:  J Bacteriol       Date:  1972-04       Impact factor: 3.490

8.  Primary structure of the Streptomyces enzyme endo-beta-N-acetylglucosaminidase H.

Authors:  P W Robbins; R B Trimble; D F Wirth; C Hering; F Maley; G F Maley; R Das; B W Gibson; N Royal; K Biemann
Journal:  J Biol Chem       Date:  1984-06-25       Impact factor: 5.157

9.  A fluorescence high-performance liquid chromatography assay for enzymes acting on the di-N-acetylchitobiosyl part of asparagine-linked glycans.

Authors:  S Bourgerie; S Berger; G Strecker; R Julien; Y Karamanos
Journal:  J Biochem Biophys Methods       Date:  1994-06

10.  Demonstration of heterogeneity of chick ovalbumin glycopeptides using 360-MHz proton magnetic resonance spectroscopy.

Authors:  P H Atkinson; A Grey; J P Carver; J Hakimi; C Ceccarini
Journal:  Biochemistry       Date:  1981-07-07       Impact factor: 3.162
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  5 in total

1.  An endo-N-acetyl-beta-D-glucosaminidase, acting on the di-N-acetylchitobiosyl part of N-linked glycans, is secreted during sporulation of Myxococcus xanthus.

Authors:  J P Barreaud; S Bourgerie; R Julien; J F Guespin-Michel; Y Karamanos
Journal:  J Bacteriol       Date:  1995-02       Impact factor: 3.490

2.  The two endo-β-N-acetylglucosaminidase genes from Arabidopsis thaliana encode cytoplasmic enzymes controlling free N-glycan levels.

Authors:  Richard M Fischl; Johannes Stadlmann; Josephine Grass; Friedrich Altmann; Renaud Léonard
Journal:  Plant Mol Biol       Date:  2011-07-28       Impact factor: 4.076

3.  AmyM, a Novel Maltohexaose-Forming α-Amylase from Corallococcus sp. strain EGB.

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Journal:  Appl Environ Microbiol       Date:  2015-01-09       Impact factor: 4.792

4.  Molecular modeling and analysis of human and plant endo-β-N-acetyl- glucosaminidases for mutations effects on function.

Authors:  Shechinah Felice Choragudi; Ganesh Kumar Veeramachaneni; Bv Raman; Bondili Js
Journal:  Bioinformation       Date:  2014-08-30

5.  Structural basis and catalytic mechanism for the dual functional endo-beta-N-acetylglucosaminidase A.

Authors:  Jie Yin; Lei Li; Neil Shaw; Yang Li; Jing Katherine Song; Wenpeng Zhang; Chengfeng Xia; Rongguang Zhang; Andrzej Joachimiak; Hou-Cheng Zhang; Lai-Xi Wang; Zhi-Jie Liu; Peng Wang
Journal:  PLoS One       Date:  2009-03-02       Impact factor: 3.240
  5 in total

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