Literature DB >> 14199018

COTTON CLOSURE AS AN AERATION BARRIER IN SHAKEN FLASK FERMENTATIONS.

J S SCHULTZ.   

Abstract

Oxygen transfer through a cotton plug follows Fick's law of diffusion. The apparent diffusion constant decreases as the density of cotton packing is increased. Formulas are derived which relate oxygen transfer to cotton-plug dimensions. Calculations indicate that the dimensions of a cotton plug can affect both the maximal oxygen uptake rate and the gas composition inside a shaken flask. In some situations, as with large flasks, the cotton closure can become the limiting factor to aeration.

Entities:  

Keywords:  BACILLUS MEGATERIUM; BACTERIOLOGICAL TECHNICS; CHLORTETRACYCLINE; COTTON; EQUIPMENT AND SUPPLIES; EXPERIMENTAL LAB STUDY; FERMENTATION; GASES; OXYGEN

Mesh：

Substances：

Year:  1964        PMID： 14199018      PMCID： PMC1058122          DOI： 10.1128/am.12.4.305-310.1964

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


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