| Literature DB >> 23296677 |
Jia Hu1, Min Wei, Mario G Mirisola, Valter D Longo.
Abstract
Saccharomyces cerevisiae is one of the most studied model organisms for the identification of genes and mechanisms that affect aging. The chronological lifespan (CLS) assay, which monitors the survival of a non-dividing population, is one of the two methods to study aging in yeast. To eliminate potential artifacts and identify genes and signaling pathways that may also affect aging in higher eukaryotes, it is important to determine CLS by multiple methods. Here, we describe these methods as well as the assays to study macromolecular damage during aging in yeast, with a focus on genomic instability.Entities:
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Year: 2013 PMID： 23296677 PMCID： PMC4041521 DOI： 10.1007/978-1-62703-239-1_30
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745