Literature DB >> 10677341

The ShBle resistance determinant from Streptoalloteichus hindustanus is expressed in Haloferax volcanii and confers resistance to bleomycin.

S D Nuttall1, S E Deutschel, R A Irving, J A Serrano-Gomicia, M L Dyall-Smith.   

Abstract

We have designed a gene cassette for expression of the bleomycin-resistance protein from Streptoalloteichus hindustanus (ShBle) in the extremely halophilic archaeon Haloferax volcanii, and shown that transformed haloarchaea are resistant to bleomycin. Recombinant ShBle was purified by a one-step affinity-chromatography procedure as a correctly folded, dimeric protein. ShBle thus provides a useful haloarchaeal selectable marker and represents the first non-halophilic and soluble heterologous protein to be expressed in the Haloarchaea.

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Year:  2000        PMID： 10677341      PMCID： PMC1220848     

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  20 in total

Review 1.  Transcription initiation in Archaea: facts, factors and future aspects.

Authors:  J Soppa
Journal:  Mol Microbiol       Date:  1999-03       Impact factor: 3.501

2.  Development of a transformation system for the thermophilic fungus Talaromyces sp. CL240 based on the use of phleomycin resistance as a dominant selectable marker.

Authors:  S Jain; H Durand; G Tiraby
Journal:  Mol Gen Genet       Date:  1992-09

3.  Mutations in DNA gyrase result in novobiocin resistance in halophilic archaebacteria.

Authors:  M L Holmes; M L Dyall-Smith
Journal:  J Bacteriol       Date:  1991-01       Impact factor: 3.490

Review 4.  DNA strand scission by activated bleomycin group antibiotics.

Authors:  S M Hecht
Journal:  Fed Proc       Date:  1986-11

5.  Mevinolin-resistant mutations identify a promoter and the gene for a eukaryote-like 3-hydroxy-3-methylglutaryl-coenzyme A reductase in the archaebacterium Haloferax volcanii.

Authors:  W L Lam; W F Doolittle
Journal:  J Biol Chem       Date:  1992-03-25       Impact factor: 5.157

6.  Dihydrofolate reductase of the extremely halophilic archaebacterium Halobacterium volcanii. The enzyme and its coding gene.

Authors:  T Zusman; I Rosenshine; G Boehm; R Jaenicke; B Leskiw; M Mevarech
Journal:  J Biol Chem       Date:  1989-11-15       Impact factor: 5.157

7.  Construction and use of halobacterial shuttle vectors and further studies on Haloferax DNA gyrase.

Authors:  M L Holmes; S D Nuttall; M L Dyall-Smith
Journal:  J Bacteriol       Date:  1991-06       Impact factor: 3.490

8.  Nuclear localization of bacterial Streptoalloteichus hindustanus bleomycin resistance protein in mammalian cells.

Authors:  T P Calmels; J S Mistry; S C Watkins; P D Robbins; R McGuire; J S Lazo
Journal:  Mol Pharmacol       Date:  1993-12       Impact factor: 4.436

9.  Transformation of members of the genus Haloarcula with shuttle vectors based on Halobacterium halobium and Haloferax volcanii plasmid replicons.

Authors:  S W Cline; W F Doolittle
Journal:  J Bacteriol       Date:  1992-02       Impact factor: 3.490

10.  Phleomycin resistance as a dominant selectable marker for plant cell transformation.

Authors:  P Perez; G Tiraby; J Kallerhoff; J Perret
Journal:  Plant Mol Biol       Date:  1989-10       Impact factor: 4.076
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  2 in total

1.  Secreted euryarchaeal microhalocins kill hyperthermophilic crenarchaea.

Authors:  C Haseltine; T Hill; R Montalvo-Rodriguez; S K Kemper; R F Shand; P Blum
Journal:  J Bacteriol       Date:  2001-01       Impact factor: 3.490

2.  Characterisation of the Dunaliella tertiolecta RbcS genes and their promoter activity in Chlamydomonas reinhardtii.

Authors:  T L Walker; D K Becker; C Collet
Journal:  Plant Cell Rep       Date:  2004-10-05       Impact factor: 4.570
  2 in total

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