Literature DB >> 2506108

Transformation of Penicillium chrysogenum with selection for increased resistance to benomyl.

T M Picknett1, G Saunders.   

Abstract

Incubation of protoplasts of Penicillium chrysogenum with the plasmid pBT-3 permitted selection of transformants on the basis of increased resistance to the anti-fungal agent benomyl. Transformants were obtained at a frequency of 1-2 per microgram of DNA. Southern analysis revealed that transformation had occurred by integration of vector sequences into the host genome.

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Year:  1989        PMID： 2506108     DOI： 10.1016/0378-1097(89)90501-6

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  5 in total

1.  Development of a transformation system for the thermophilic fungus Talaromyces sp. CL240 based on the use of phleomycin resistance as a dominant selectable marker.

Authors:  S Jain; H Durand; G Tiraby
Journal:  Mol Gen Genet       Date:  1992-09

2.  Genetic Transformation of the Biocontrol Fungus Gliocladium virens to Benomyl Resistance.

Authors:  N Ossanna; S Mischke
Journal:  Appl Environ Microbiol       Date:  1990-10       Impact factor: 4.792

3.  Development of a new transformant selection system for Penicillium chrysogenum: isolation and characterization of the P. chrysogenum acetyl-coenzyme A synthetase gene (facA) and its use as a homologous selection marker.

Authors:  R J Gouka; W van Hartingsveldt; R A Bovenberg; C M van Zeijl; C A van den Hondel; R F van Gorcom
Journal:  Appl Microbiol Biotechnol       Date:  1993-01       Impact factor: 4.813

Review 4.  Penicillium chrysogenum, a Vintage Model with a Cutting-Edge Profile in Biotechnology.

Authors:  Francisco Fierro; Inmaculada Vaca; Nancy I Castillo; Ramón Ovidio García-Rico; Renato Chávez
Journal:  Microorganisms       Date:  2022-03-06

5.  Transformation of Penicillium rubens 212 and Expression of GFP and DsRED Coding Genes for Visualization of Plant-Biocontrol Agent Interaction.

Authors:  Maria Villarino; Eduardo A Espeso; Paloma Melgarejo; Inmaculada Larena
Journal:  Front Microbiol       Date:  2018-07-23       Impact factor: 5.640
  5 in total

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