Literature DB >> 1394517

A ten-minute protocol for transforming Saccharomyces cerevisiae by electroporation.

M Grey1, M Brendel.   

Abstract

We present a simplified and rapid method for the transformation of yeast cells by electroporation. Stationary cells, scraped off the agar of Petri dish cultures stored in the refrigerator for up to 6 weeks, are suspended in sorbitol buffer, spun down by gentle centrifugation, transferred into the electroporation cuvette, and immediately subjected to transformation via electroporation. Transformation efficiency of this 10-min method, which does not require the preparation of cell cultures, is about 10% of the hitherto best performing transformation procedure using cells of defined growth phase.

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Year:  1992        PMID： 1394517     DOI： 10.1007/bf00317931

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  8 in total

1.  High-efficiency transformation of yeast by electroporation.

Authors:  D M Becker; L Guarente
Journal:  Methods Enzymol       Date:  1991       Impact factor: 1.600

2.  High efficiency transformation of E. coli by high voltage electroporation.

Authors:  W J Dower; J F Miller; C W Ragsdale
Journal:  Nucleic Acids Res       Date:  1988-07-11       Impact factor: 16.971

3.  Yeast/E. coli shuttle vectors with multiple unique restriction sites.

Authors:  J E Hill; A M Myers; T J Koerner; A Tzagoloff
Journal:  Yeast       Date:  1986-09       Impact factor: 3.239

4.  Transformation of Saccharomyces cerevisiae by electroporation.

Authors:  E Delorme
Journal:  Appl Environ Microbiol       Date:  1989-09       Impact factor: 4.792

5.  Electroporation of eukaryotes and prokaryotes: a general approach to the introduction of macromolecules into cells.

Authors:  K Shigekawa; W J Dower
Journal:  Biotechniques       Date:  1988-09       Impact factor: 1.993

6.  Expression of genes transferred into monocot and dicot plant cells by electroporation.

Authors:  M Fromm; L P Taylor; V Walbot
Journal:  Proc Natl Acad Sci U S A       Date:  1985-09       Impact factor: 11.205

7.  Electric field mediated gene transfer.

Authors:  T K Wong; E Neumann
Journal:  Biochem Biophys Res Commun       Date:  1982-07-30       Impact factor: 3.575

8.  Gene transfer into mouse lyoma cells by electroporation in high electric fields.

Authors:  E Neumann; M Schaefer-Ridder; Y Wang; P H Hofschneider
Journal:  EMBO J       Date:  1982       Impact factor: 11.598
  8 in total
  12 in total

1.  Transformation of Kluyveromyces lactis by Electroporation.

Authors:  M Sánchez; F J Iglesias; C Santamaría; A Domínguez
Journal:  Appl Environ Microbiol       Date:  1993-07       Impact factor: 4.792

2.  Random mutagenesis reveals a region important for gating of the yeast K+ channel Ykc1.

Authors:  S H Loukin; B Vaillant; X L Zhou; E P Spalding; C Kung; Y Saimi
Journal:  EMBO J       Date:  1997-08-15       Impact factor: 11.598

3.  Hypotonic shocks activate rat TRPV4 in yeast in the absence of polyunsaturated fatty acids.

Authors:  Stephen H Loukin; Zhenwei Su; Ching Kung
Journal:  FEBS Lett       Date:  2009-01-25       Impact factor: 4.124

4.  Isolation and structure of an acetolactate synthase gene from Schizosaccharomyces pombe and complementation of the ilv2 mutation in Saccharomyces cerevisiae.

Authors:  F Bekkaoui; S A Nadin-Davis; W L Crosby
Journal:  Curr Genet       Date:  1993-12       Impact factor: 3.886

5.  Accumulation of mRNA coding for the ctf13p kinetochore subunit of Saccharomyces cerevisiae depends on the same factors that promote rapid decay of nonsense mRNAs.

Authors:  J N Dahlseid; J Puziss; R L Shirley; A L Atkin; P Hieter; M R Culbertson
Journal:  Genetics       Date:  1998-11       Impact factor: 4.562

6.  Nonsense-mediated decay of ash1 nonsense transcripts in Saccharomyces cerevisiae.

Authors:  Wei Zheng; Jonathan S Finkel; Sharon M Landers; Roy M Long; Michael R Culbertson
Journal:  Genetics       Date:  2008-09-14       Impact factor: 4.562

7.  Genetic background affects relative nonsense mRNA accumulation in wild-type and upf mutant yeast strains.

Authors:  Bessie Kebaara; Tara Nazarenus; Rachel Taylor; Audrey L Atkin
Journal:  Curr Genet       Date:  2003-04-15       Impact factor: 3.886

8.  Identification of antifungal natural products via Saccharomyces cerevisiae bioassay: insights into macrotetrolide drug spectrum, potency and mode of action.

Authors:  Brad Tebbets; Zhiguo Yu; Douglas Stewart; Li-Xing Zhao; Yi Jiang; Li-Hua Xu; David Andes; Ben Shen; Bruce Klein
Journal:  Med Mycol       Date:  2012-08-28       Impact factor: 4.076

9.  Programmed frameshifting in the synthesis of mammalian antizyme is +1 in mammals, predominantly +1 in fission yeast, but -2 in budding yeast.

Authors:  I P Ivanov; R F Gesteland; S Matsufuji; J F Atkins
Journal:  RNA       Date:  1998-10       Impact factor: 4.942

10.  The Upf-dependent decay of wild-type PPR1 mRNA depends on its 5'-UTR and first 92 ORF nucleotides.

Authors:  B Kebaara; T Nazarenus; R Taylor; A Forch; A L Atkin
Journal:  Nucleic Acids Res       Date:  2003-06-15       Impact factor: 16.971
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